Supplementary Materials

Supplementary Material for:

Loss of GPR3 reduces the amyloid plaque burden and improves memory in Alzheimer?s disease mouse models

Yunhong Huang, Aneta Skwarek-Maruszewska, Katrien Horré, Elke Vandewyer, Leen Wolfs, An Snellinx, Takashi Saito, Enrico Radaelli, Nikky Corthout, Julien Colombelli, Adrian C. Lo, Leen Van Aerschot, Zsuzsanna Callaerts-Vegh, Daniah Trabzuni, Koen Bossers, Joost Verhaagen, Mina Ryten, Sebastian Munck, Rudi D’Hooge, Dick F. Swaab, John Hardy, Takaomi C. Saido, Bart De Strooper,* Amantha Thathiah*

*Corresponding author. E-mail: amantha.thathiah{at}cme.vib-kuleuven.be (A.T.); bart.destrooper{at}cme.vibkuleuven. (B.D.S.)

Published 14 October 2015, Sci. Transl. Med. 7, 309ra164 (2015)
DOI: 10.1126/scitranslmed.aab3492

This PDF file includes:

  • Fig. S1. The Aβ42/Aβ40 ratio in AD transgenic mouse models.
  • Fig. S2. Notch signaling in APP/PS1;Gpr3−/− mice relative to APP/PS1;Gpr3+/+ mice.
  • Fig. S3. APP expression levels in the brains of wild-type (Appwt/wt), AppNL/NL;Gpr3+/+, AppNL/NL;Gpr3−/−, AppNL-F/NL-F;Gpr3+/+, and AppNL-F/NL-F;Gpr3−/− mice.
  • Fig. S4. Thioflavin-S and Aβ antibody (6E10) staining in APP/PS1 mice.
  • Fig. S5. Behavioral studies in APP/PS1;Gpr3+/+, APP/PS1;Gpr3+/−, and APP/PS1;Gpr3−/− mice.
  • Fig. S6. Behavioral studies in Gpr3+/+ and Gpr3−/− mice.
  • Fig. S7. The mRNA expression of GPR3 in the hippocampus and cerebral cortex of the normal murine brain.
  • Fig. S8. Changes in glial markers in AD patients.
  • Table S1. Exploratory behavior in AD transgenic mice crossed with Gpr3+/−and Gpr3−/− mice and in nontransgenic mice.
  • Table S2. Mean Aβ levels in different mouse models.

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Other Supplementary Material for this manuscript includes the following:

  • Table S3 (Microsoft Excel format). Source data.