Supplementary Materials

Supplementary Material for:

Smooth muscle cell progenitors are primed to muscularize in pulmonary hypertension

Abdul Q. Sheikh, Ashish Misra, Ivan O. Rosas, Ralf H. Adams, Daniel M. Greif*

*Corresponding author. E-mail: daniel.greif{at}yale.edu

Published 7 October 2015, Sci. Transl. Med. 7, 308ra159 (2015)
DOI: 10.1126/scitranslmed.aaa9712

This PDF file includes:

  • Methods
  • Fig. S1. Specific distal pulmonary arterioles muscularize with hypoxia.
  • Fig. S2. Hypoxia exposure in mice induces KLF4 expression in PDGFR-β+ pulmonary arteriole SMCs.
  • Fig. S3. Smooth muscle Klf4 deletion in SMA-CreER, Klf4(flox/flox) mice.
  • Fig. S4. PDGFR-β+SMA+SMMHC+ cells are located at the new distally located muscular-unmuscular vascular transition zone at hypoxia day 21.
  • Fig. S5. In human PA SMCs, KLF4 regulates hypoxia- and PDGF-BB–induced migration and hypoxia-induced migration.
  • Fig. S6. Mosaic analysis of the role of KLF4 in distal pulmonary arteriole muscularization.
  • Fig. S7. With hypoxic exposure, lung PDGF-B protein expression is enhanced and then polarized and finally down-regulated.
  • Fig. S8. Lung ECs isolated from mice exposed to hypoxia have enhanced Pdgf-b expression.
  • Table S1. Primer pair sequences for quantitative reverse transcription polymerase chain reaction.
  • Reference (40)

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