Supplementary Materials

Supplementary Material for:

Human endogenous retrovirus-K contributes to motor neuron disease

Wenxue Li, Myoung-Hwa Lee, Lisa Henderson, Richa Tyagi, Muzna Bachani, Joseph Steiner, Emilie Campanac, Dax A. Hoffman, Gloria von Geldern, Kory Johnson, Dragan Maric, H. Douglas Morris, Margaret Lentz, Katherine Pak, Andrew Mammen, Lyle Ostrow, Jeffrey Rothstein, Avindra Nath*

*Corresponding author. E-mail: natha{at}ninds.nih.gov

Published 30 September 2015, Sci. Transl. Med. 7, 307ra153 (2015)
DOI: 10.1126/scitranslmed.aac8201

This PDF file includes:

  • Materials and Methods
  • Fig. S1. HERV-K expression in autopsied human brain.
  • Fig. S2. Induction of HERV-K expression in human neurons.
  • Fig. S3. Effect of HERV-K env in postnatal neurons of mice.
  • Fig. S4. Expression of HERV-K env in the brain of transgenic mice.
  • Fig. S5. Distribution of cellular proteins in wild-type and HERV-K env transgenic mouse brains.
  • Fig. S6. Changes in spine morphology and dendritic damage in pyramidal neurons in HERV-K env transgenic mice.
  • Fig. S7. Magnetic resonance imaging and regional analysis of mouse brains.
  • Fig. S8. Muscle pathology in HERV-K env transgenic mice.
  • Fig. S9. Impairment of locomotor behavior in HERV-K env transgenic mice.
  • Fig. S10. TDP-43 binds to the HERV-K LTR in vivo, and TDP-43 binding to HERV-K LTR correlates with the association of a processive RNA polymerase II (phosphorylated Ser2) on the LTR.
  • Table S1. Clinical data for the human brain samples.
  • Table S2. Passive and active membrane properties of medial prefrontal cortex layer V pyramidal neurons.
  • Table S3. Primer sequences.
  • Legend for movie S1
  • References (3548)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Hindlimb clasping in a tail suspension test. (A) Control. (B) Transgenic animal.

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