Supplementary Materials

Supplementary Material for:

Early infancy microbial and metabolic alterations affect risk of childhood asthma

Marie-Claire Arrieta, Leah T. Stiemsma, Pedro A. Dimitriu, Lisa Thorson, Shannon Russell, Sophie Yurist-Doutsch, Boris Kuzeljevic, Matthew J. Gold, Heidi M. Britton, Diana L. Lefebvre, Padmaja Subbarao, Piush Mandhane, Allan Becker, Kelly M. McNagny, Malcolm R. Sears, Tobias Kollmann, the CHILD Study Investigators, William W. Mohn, Stuart E. Turvey,* B. Brett Finlay*

*Corresponding author. E-mail: sturvey{at}cw.bc.ca (S.E.T.); bfinlay{at}mail.ubc.ca (B.B.F.)

Published 30 September 2015, Sci. Transl. Med. 7, 307ra152 (2015)
DOI: 10.1126/scitranslmed.aab2271

This PDF file includes:

  • Fig. S1. Gut microbial and host metabolic changes in the first year of life.
  • Fig. S2. Human gut microbiota at 3 months and 1 year of age.
  • Fig. S3. One-year-old gut microbiota by clinical phenotype.
  • Fig. S4. Relative abundance of bacterial genera at (A) 3 months and (B) 1 year.
  • Fig. S5. PICRUSt-predicted KEGG functional categories.
  • Fig. S6. Three-month gut microbiota classified by (A) wheeze/non-wheeze or (B) atopy/non-atopy.
  • Fig. S7. One-year gut microbiota classified by (A) wheeze/non-wheeze or (B) atopy/non-atopy.
  • Fig. S8. Microbial-derived functional changes in mice treated with FLVR.
  • Fig. S9. Heatmap of a biweight correlation (bicor) between BAL total cell counts and the top 30 bacterial OTUs from F1 mice feces at 3 weeks of age.
  • Fig. S10. Lung cytokine concentration and serum concentration of OVA-specific antibodies.
  • Table S1. Characteristics of the cohort.
  • Table S2. Differentially abundant OTUs between 3-month and 1-year samples.
  • Table S3. Differentially abundant taxa among the four clinical phenotypes at 3 months and 1 year.
  • Table S4. PICRUSt-predicted top 30 differential KOs (based on P value) in AW and controls at 3 months and 1 year.
  • Table S5. Top 30 biochemical pathways (based on P value) of PICRUSt-predicted KOs in AW and controls at 3 months.
  • Table S6. SCFAs in feces and urine in AW and controls at 3 months and 1 year.
  • Table S7. 16S V3 region amplification primers and barcodes.
  • Table S8. qPCR primer sequences for selected bacterial genera.
  • Table S9. Exact logistic regression model of qPCR control subset.
  • Table S10. Exact logistic regression model of metabolomics control subset.
  • Table S11. Exact logistic regression model of SCFA control subset.
  • Table S12. Exact logistic regression model of metabolomics AW subset*.
  • Table S13. Exact logistic regression model of SCFA AW subset*.
  • Legends for additional data tables

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Other Supplementary Material for this manuscript includes the following:

  • Additional Data Tables (separate file)
  • Additional Data Table S1 (Microsoft Excel format). PICRUSt-predicted differentially abundant KOs between AW and controls.
  • Additional Data Table S2 (Microsoft Excel format). PICRUSt-predicted differentially abundant pathways between AW- and AW + FLVR–treated mice.
  • Additional Data Table S3 (Microsoft Excel format). Raw data and P values for all experiments with n < 20.

[Download Additional Data Tables]