Supplementary Materials

Supplementary Material for:

Effects of caffeine on the human circadian clock in vivo and in vitro

Tina M. Burke, Rachel R. Markwald, Andrew W. McHill, Evan D. Chinoy, Jesse A. Snider, Sara C. Bessman, Christopher M. Jung, John S. O'Neill,* Kenneth P. Wright Jr.*

*Corresponding author. E-mail: kenneth.wright{at}colorado.edu (K.P.W.); oneillj{at}mrc-lmb.cam.ac.uk (J.S.O.)

Published 16 September 2015, Sci. Transl. Med. 7, 305ra146 (2015)
DOI: 10.1126/scitranslmed.aac5125

This PDF file includes:

  • Fig. S1. Forty-nine day protocol for human experiment.
  • Fig. S2. Ryanodine does not lengthen the circadian period reported by per2:luc in cultured human cells in vitro.
  • Fig. S3. Caffeine increases the circadian period reported by bmal1:luc in cultured human cells in vitro in an adenosine receptor/cAMP–dependent fashion.
  • Fig. S4. Caffeine increases the circadian period reported by per2:luc in cultured human cells in vitro in an adenosine receptor/cAMP–dependent fashion.
  • Fig. S5. Caffeine acts at the same site as 8-SPT to increase the circadian period in cultured human cells in vitro.
  • Fig. S6. Quantification of adenosine receptor siRNA knockdown efficacy.

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