Supplementary Materials

Supplementary Material for:

Dengue virus NS1 triggers endothelial permeability and vascular leak that is prevented by NS1 vaccination

P. Robert Beatty, Henry Puerta-Guardo, Sarah S. Killingbeck, Dustin R. Glasner, Kaycie Hopkins, Eva Harris*

*Corresponding author. E-mail: eharris{at}berkeley.edu

Published 9 September 2015, Sci. Transl. Med. 7, 304ra141 (2015)
DOI: 10.1126/scitranslmed.aaa3787

This PDF file includes:

  • Fig. S1. Recombinant NS1 from DENV1, DENV2, DENV3, DENV4, and WNV is highly purified and oligomeric.
  • Fig. S2. Morbidity and vascular leakage are induced by NS1.
  • Fig. S3. NS1 increases endothelial cell permeability in two different endothelial cell lines, HUVEC and HPMEC.
  • Fig. S4. Viremia after lethal DENV challenge is reduced by immunization with DENV2 NS1.
  • Fig. S5. Immunization with DENV NS1 and DENV infection induces serotype cross-reactive anti-NS1 antibodies.

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