Supplementary Materials

Supplementary Material for:

Targeting Siglecs with a sialic acid–decorated nanoparticle abrogates inflammation

Shaun Spence, Michelle K. Greene, François Fay, Emily Hams, Sean P. Saunders, Umar Hamid, Marianne Fitzgerald, Jonathan Beck, Baljinder K. Bains, Peter Smyth, Efrosyni Themistou, Donna M. Small, Daniela Schmid, Cecilia M. O'Kane, Denise C. Fitzgerald, Sharif M. Abdelghany, James A. Johnston, Padraic G. Fallon, James F. Burrows, Daniel F. McAuley, Adrien Kissenpfennig, Christopher J. Scott*

*Corresponding author. E-mail: c.scott{at}qub.ac.uk

Published 2 September 2015, Sci. Transl. Med. 7, 303ra140 (2015)
DOI: 10.1126/scitranslmed.aab3459

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Nanoparticle characterization.
  • Fig. S2. α2,8 NANA-NP bind to Siglec-E and induce receptor clustering.
  • Fig. S3. Clodronate depletion of macrophages.
  • Fig. S4. Differential expression of Siglec-E and uptake of fluorescent α2,8 NANA-NP by macrophages and neutrophils.
  • Fig. S5. α2,8 NANA-NP exhibit no toxicity in preclinical models.
  • Fig. S6. α2,8 NANA-NP prevent IκB degradation in THP-1 cells and enhance IL-10 production in the EVLP model.
  • Fig. S7. Schematic of EVLP model and proposed mechanism of action of α2,8 NANA-NP.

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