Supplementary Materials

Supplementary Material for:

Quality and quantity of TFH cells are critical for broad antibody development in SHIVAD8 infection

Takuya Yamamoto, Rebecca M. Lynch, Rajeev Gautam, Rodrigo Matus-Nicodemos, Stephen D. Schmidt, Kristin L. Boswell, Sam Darko, Patrick Wong, Zizhang Sheng, Constantinos Petrovas, Adrian B. McDermott, Robert A. Seder, Brandon F. Keele, Lawrence Shapiro, Daniel C. Douek, Yoshiaki Nishimura, John R. Mascola, Malcolm A. Martin, Richard A. Koup*

*Corresponding author. E-mail: rkoup{at}

Published 29 July 2015, Sci. Transl. Med. 7, 298ra120 (2015)
DOI: 10.1126/scitranslmed.aab3964

This PDF file includes:

  • Fig. S1. Gating scheme for TFH.
  • Fig. S2. Plasma sCD14 levels and IL-6 levels of RMs.
  • Fig. S3. Longitudinal development of NAbs during SHIVAD8 infection.
  • Fig. S4. Representative gating scheme used for the quantification of Env-specific B cells in an LN.
  • Fig. S5. Total TFH and IgG+ B cells.
  • Fig. S6. Quantification of HIV Gag and Env-specific TFH cells.
  • Fig. S7. Antigen-specific responses in the LN.
  • Fig. S8. Gating scheme for Bcl6 expression in TFH cells.
  • Fig. S9. Gene signatures of Env-specific CD154+ TFH cells.
  • Fig. S10. Expansion of highly mutated clonal families of gp140-specific B cells.
  • Fig. S11. Neutralization by affinity-matured antibodies.
  • Fig. S12. Epitope mapping of JG7-VRC30 family of mAbs.
  • Fig. S13. The frequency of peripheral TFH (pTFH)–like cells does not predict broad neutralization activity.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Source data.

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