Supplementary Materials

Supplementary Material for:

Human NK cell repertoire diversity reflects immune experience and correlates with viral susceptibility

Dara M. Strauss-Albee, Julia Fukuyama, Emily C. Liang, Yi Yao, Justin A. Jarrell, Alison L. Drake, John Kinuthia, Ruth R. Montgomery, Grace John-Stewart, Susan Holmes, Catherine A. Blish*

*Corresponding author. E-mail: cblish{at}stanford.edu

Published 22 July 2015, Sci. Transl. Med. 7, 297ra115 (2015)
DOI: 10.1126/scitranslmed.aac5722

This PDF file includes:

  • Fig. S1. Serial negative gating strategy used to define NK, CD4+ T, and CD8+ T cells from PBMCs.
  • Fig. S2. Representative gates to evaluate marker expression on NK cells.
  • Fig. S3. Stability analysis of CD56bright versus CD56dim NK cells.
  • Fig. S4. Human NK cell repertoire and function are stable for 6 months in an individual.
  • Fig. S5. Subpopulation analysis for the 10 most frequently detected NK subpopulations.
  • Fig. S6. Donor age does not correlate with NK cell diversity.
  • Fig. S7. CMV serostatus does not correlate with NK cell diversity or the frequency of NKG2A or CD57.
  • Fig. S8. NK diversity does not correlate with viral suppression in vitro.
  • Fig. S9. No correlative features distinguish NK cells responding to HIV-1–infected CD4+ T cells.
  • Fig. S10. Cytokine-producing NK cells are more diverse than non–cytokine-producing NK cells.
  • Fig. S11. Proportion of variance explained in correspondence analysis.
  • Table S1. Mass cytometry antibody panels used in each experiment.
  • Table S2. HIP and SBB cohort information.
  • Table S3. Mama Salama Study cohort information.
  • Reference (48)

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Other Supplementary Material for this manuscript includes the following:

  • Table S4 (Microsoft Excel format). Frequencies of NK markers used in repertoire stability analysis.
  • Table S5 (Microsoft Excel format). Frequencies of NK markers after 72-hour culture with IL-15 or IL-2, or without stimulation, for the SBB cohort.
  • Table S6 (Microsoft Excel format). Frequencies of functional markers used in stability analysis.
  • Table S7 (Microsoft Excel format). CD57 and NKG2A frequencies and NK diversity scores for HIP and cord blood cohorts.
  • Table S8 (Microsoft Excel format). Proportion of phenotypes expressing CD57 and NKG2A.
  • Table S9 (Microsoft Excel format). Functional frequencies of CD57+ and CD57NK cells.
  • Table S10 (Microsoft Excel format). Diversity of NK cells in the presence or absence of HIV-1–infected CD4+ T cells.
  • Table S11 (Microsoft Excel format). Diversity of NK cells in the presence or absence of WNV-infected cells.

[Download Tables S4 to S11]