Supplementary Materials

Supplementary Material for:

HLA class II genes modulate vaccine-induced antibody responses to affect HIV-1 acquisition

Heather A. Prentice, Georgia D. Tomaras, Daniel E. Geraghty, Richard Apps, Youyi Fong, Philip K. Ehrenberg, Morgane Rolland, Gustavo H. Kijak, Shelly J. Krebs, Wyatt Nelson, Allan DeCamp, Xiaoying Shen, Nicole L. Yates, Susan Zolla-Pazner, Sorachai Nitayaphan, Supachai Rerks-Ngarm, Jaranit Kaewkungwal, Punnee Pitisuttithum, Guido Ferrari, M. Juliana McElrath, David C. Montefiori, Robert T. Bailer, Richard A. Koup, Robert J. O'Connell, Merlin L. Robb, Nelson L. Michael, Peter B. Gilbert, Jerome H. Kim, Rasmi Thomas*

*Corresponding author. E-mail: rthomas{at}hivresearch.org

Published 15 July 2015, Sci. Transl. Med. 7, 296ra112 (2015)
DOI: 10.1126/scitranslmed.aab4005

This PDF file includes:

  • Fig. S1. Multiple alignment of different Env (120–204) and partial V2 sequences (160–183).
  • Fig. S2. Cocrystal structure of HIV-1 gp120 (red) binding to CD4 (green) and the Fab of mAb 17b (blue) (23).
  • Table S1. Odds ratios for two-way interaction analysis of HLA class II alleles with Env-specific IgA and Env (120–204)–specific IgG.
  • Table S2. Association between Env-specific IgA or Env (120–204)–specific IgG and HLA class II alleles present in more than 5% of the study population.
  • Table S3. High IgG binding to multiple Env (120–204) antigens correlates with presence of DPB1*13 and is associated with decreased HIV-1 acquisition across
    multiple subtypes.
  • Table S4. IgG responses to five overlapping peptides spanning Env (120–204) were significantly associated with presence of DPB1*13.
  • Table S5. Comparison of frequency of amino acid (AA) sites in Env-gp70 in RV144 breakthrough infections stratified by DPB1*13.
  • Table S6. Odds ratios for IgA binding to different recombinant Env proteins on HIV-1 acquisition after stratification by absence or presence of HLA-DQB1*06.
  • Table S7. Odds ratios for HIV-1 acquisition in univariate analyses of all HLA class II alleles present in the placebo controls.
  • Table S8. Odds ratios for HIV-1 acquisition in univariate analyses of all HLA class II alleles present in the vaccinated volunteers.

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