Supplementary Materials

Supplementary Material for:

Vitamin B12 modulates the transcriptome of the skin microbiota in acne pathogenesis

Dezhi Kang, Baochen Shi, Marie C. Erfe, Noah Craft, Huiying Li*

*Corresponding author. E-mail: huiying{at}mednet.ucla.edu

Published 24 June 2015, Sci. Transl. Med. 7, 293ra103 (2015)
DOI: 10.1126/scitranslmed.aab2009

This PDF file includes:

  • Materials and Methods
  • Fig. S1. The metatranscriptome composition of the skin microbiota in follicles.
  • Fig. S2. The KEGG pathways expressed in all the samples.
  • Fig. S3. The KEGG pathways with differentially expressed P. acnes OGUs.
  • Fig. S4. The cob/cbi operons in P. acnes.
  • Fig. S5. Differentially expressed P. acnes OGUs in sample HL414_Day14 compared to the other day 14 samples.
  • Fig. S6. The biosynthesis of porphyrins is inversely correlated with the biosynthesis of vitamin B12 in P. acnes.
  • Fig. S7. A comparison of the samples collected from opposite sides of the nose of the same individual using the Bioré strip method and the tape stripping method.
  • Fig. S8. Rarefaction curves indicate sufficient sequencing depths of the samples.
  • Table S1. High sequencing depths of the metatranscriptomic data.
  • Table S2. Differentially expressed OGUs shown in Fig. 1B.
  • Table S3. Full names of the genes and substrates shown in Fig. 2.
  • Table S4. The relative expression levels (log10) of the vitamin B12 biosynthesis genes in the skin microbiota of the healthy individuals and acne patients shown in
    Fig. 3.
  • Table S5A. The relative expression levels (log10) of the vitamin B12 biosynthesis genes in the skin microbiota of the healthy subjects shown in Fig. 4.
  • Table S5B. The relative expression levels (log10) of the vitamin B12 biosynthesis genes in the skin microbiota of the acne patients shown in Fig. 4.
  • Table S6. The differentially expressed OGUs in both the comparison between sample HL414_Day14 and other day 14 samples and the comparison between day
    0 and day 14 samples in the vitamin B12 supplementation study.
  • Table S7. The relative expression levels (log10) of the vitamin B12 biosynthesis genes in the P. acnes cultures shown in Fig. 6A.
  • Table S8. The porphyrin levels detected in the P. acnes cultures shown in Fig. 6B.
  • References (7482)

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