Supplementary Materials

Supplementary Material for:

Vinculin network–mediated cytoskeletal remodeling regulates contractile function in the aging heart

Gaurav Kaushik, Alice Spenlehauer, Ayla O. Sessions, Adriana S. Trujillo, Alexander Fuhrmann, Zongming Fu, Vidya Venkatraman, Danielle Pohl, Jeremy Tuler, Mingyi Wang, Edward G. Lakatta, Karen Ocorr, Rolf Bodmer, Sanford I. Bernstein, Jennifer E. Van Eyk, Anthony Cammarato,* Adam J. Engler*

*Corresponding author. E-mail: acammar3{at}jhmi.edu (A.C.); aengler{at}ucsd.edu (A.J.E.)

Published 17 June 2015, Sci. Transl. Med. 7, 292ra99 (2015)
DOI: 10.1126/scitranslmed.aaa5843

This PDF file includes:

  • Materials and Methods
  • Fig. S1. STRAP analysis of gene ontology and biological function for simian and murine proteomes.
  • Fig. S2. Partial interaction map of upstream vinculin regulators.
  • Fig. S3. Biometric comparison of adult and aged rats.
  • Fig. S4. Analysis of vinculin localization in rat and fly myocytes.
  • Fig. S5. Vinculin structure and homology across monkey, rat, and fly.
  • Fig. S6. Indentation of isolated adult and aged rat cardiomyocytes and intact fly hearts.
  • Fig. S7. Effects of heart tube preparation on its function.
  • Fig. S8. Characterization of diastolic diameter, cardiac stiffness, and vinculin expression in the wCS Drosophila genotype.
  • Fig. S9. Fitting shortening velocities with Hill’s muscle model.
  • Fig. S10. Generation of the UAS-Mhc RNAi;UAS-Vinc line.
  • Fig. S11. Quantification of genetic perturbations in Drosophila hearts.
  • Fig. S12. Change in VincHE stiffness with cytoskeletal perturbation.
  • Fig. S13. Transgenic fly heart rate, period, and rate variance.
  • Fig. S14. Analysis of interfilament spacing in TEM images from the Drosophila heart.
  • Legends for tables S1 to S13
  • Reference (47)

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Peptides detected by mass spectroscopy for adult and aged rhesus monkey left ventricles.
  • Table S2 (Microsoft Excel format). Peptides detected by mass spectroscopy for adult and aged rat left ventricles.
  • Table S3 (Microsoft Excel format). Proteomic analysis for adult and aged rhesus monkey left ventricles.
  • Table S4 (Microsoft Excel format). Proteomic analysis for adult and aged rat left ventricles.
  • Table S5 (Microsoft Excel format). STRAP annotation of the cellular compartments of proteins detected in both rat and monkey proteomes.
  • Table S6 (Microsoft Excel format). STRAP annotation of biological functions for rat and monkey.
  • Table S7 (Microsoft Excel format). IPA of bio-function expression for rat.
  • Table S8 (Microsoft Excel format). IPA of tox-function expression for rat.
  • Table S9 (Microsoft Excel format). IPA and OMIM annotation of age–upregulated proteins associated with cardiac function.
  • Table S10 (Microsoft Excel format). IPA of upstream regulators of age-related proteins identified in rat and simian.
  • Table S11 (Microsoft Excel format). Expression of candidate actin-binding molecules in Drosophila hearts using qPCR.
  • Table S12 (Microsoft Excel format). Fitting shortening velocities with Hill’s muscle model.
  • Table S13 (Microsoft Excel format). qPCR primers.

[Download Tables S1 to S13]