Supplementary Materials

Supplementary Material for:

Genetic variants associated with autoimmunity drive NFκB signaling and responses to inflammatory stimuli

William J. Housley, Salvador D. Fernandez, Kenneth Vera, Sasidhar R. Murikinati, Jaime Grutzendler, Nicole Cuerdon, Laura Glick, Phillip L. De Jager, Mitja Mitrovic, Chris Cotsapas, David A. Hafler*

*Corresponding author. E-mail: david.hafler{at}yale.edu

Published 10 June 2015, Sci. Transl. Med. 7, 291ra93 (2015)
DOI: 10.1126/scitranslmed.aaa9223

This PDF file includes:

  • Fig. S1. Confirmation of increased pNFκB in MS patients.
  • Fig. S2. Gating strategy and representative histogram for rs228614 GG (risk) and AA (protective) variants.
  • Fig. S3. rs228614 and rs7665090 proximal to NFκB1 result in increased IκBα degradation after TNFα treatment.
  • Fig. S4. Constitutive total IκBα and phospho-p65 NFκB are similar in rs228614 risk and protective variants.
  • Fig. S5. Full uncut gel for Fig. 4.
  • Fig. S6. TNFa and PMA responses are stable across multiple draws.
  • Fig. S7. Responses to TNFa do not change with age, gender, or ethnicity.
  • Fig. S8. TNFa signals through TNFR1 on naïve CD4 cells.
  • Fig. S9. The rs1800693 CC variant does not change the surface expression of TNFR1.
  • Fig. S10. Representative single-stained control.
  • Table S1. Demographics of healthy subjects and MS samples for Fig. 1 and fig. S1.
  • Table S2. Demographics of rs228614 results from Fig. 2.
  • Table S3. Demographics of rs7665090 from Fig. 2.
  • Table S4. Demographics of rs1800693 from Fig. 5.

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Other Supplementary Material for this manuscript includes the following:

  • Table S5 (Microsoft Excel format). Luminex data from rs1800693 subjects.
  • Table S6 (Microsoft Excel format). Raw data.

[Download Table S5 and S6]