Supplementary Materials

Supplementary Material for:

SLC7A11 expression is associated with seizures and predicts poor survival in patients with malignant glioma

Stephanie M. Robert, Susan C. Buckingham, Susan L. Campbell, Stefanie Robel, Kenneth T. Holt, Toyin Ogunrinu-Babarinde, Paula P. Warren, David M. White, Meredith A. Reid, Jenny M. Eschbacher, Michael E. Berens, Adrienne C. Lahti, Louis B. Nabors, Harald Sontheimer*

*Corresponding author. E-mail: sontheimer{at}uab.edu

Published 27 May 2015, Sci. Transl. Med. 7, 289ra86 (2015)
DOI: 10.1126/scitranslmed.aaa8103

This PDF file includes:

  • Materials and Methods
  • Fig. S1. SLC7A11 expression is increased in the tumor edge.
  • Fig. S2. SLC7A11 expression is stable in the PDX-GBM xenoline glioma model.
  • Fig. S3. Medium from SXC-expressing gliospheres causes intracellular Ca2+ increases in neurons.
  • Fig. S4. SXC-mediated glutamate release causes neuronal death by glutamate receptor overstimulation.
  • Fig. S5. SXC-expressing tumors induce peritumoral neuronal cell loss.
  • Fig. S6. Contralateral brain has abundant NeuN+ neurons and GFAP+ astrocytes.
  • Fig. S7. Intracranially injected astrocytes do not cause neuronal cell loss or change in SXC expression of surrounding tissue.
  • Fig. S8. SXC-expressing gliomas proliferate faster and are more invasive.
  • Fig. S9. SXC-expressing gliomas demonstrate an invasive phenotype.
  • Fig. S10. Peritumoral neurons have similar action potential thresholds to neurons in sham-injected control cortex.
  • Fig. S11. SXC is variably expressed in different grades of glioma.
  • Fig. S12. MRS detection of glutamate is reproducible and shows rebound peak after SAS administration.
  • Fig. S13. EEG traces from glioma patients show abnormal activity.
  • Table S1. Demographic and clinical data for patients in MRS clinical trial.
  • Legend for movie S1
  • References (5761)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Original data and exact P values.
  • Movie S1 (.mp4 format). Seizures induced by SXC-expressing tumors are apparent on video monitoring.

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[Download Movie S1]