Supplementary Materials

Supplementary Material for:

Programmable probiotics for detection of cancer in urine

Tal Danino, Arthur Prindle, Gabriel A. Kwong, Matthew Skalak, Howard Li, Kaitlin Allen, Jeff Hasty, Sangeeta N. Bhatia*

*Corresponding author. E-mail: sbhatia{at}

Published 27 May 2015, Sci. Transl. Med. 7, 289ra84 (2015)
DOI: 10.1126/scitranslmed.aaa3519

This PDF file includes:

  • Fig. S1. Map of plasmid pTKW106 alp7A.
  • Fig. S2. Size and colonization of liver metastases.
  • Fig. S3. Calibration curve for the qPCR calculation of the number bacteria in a tumor sample.
  • Fig. S4. Histopathological analysis of organs after oral delivery of PROP-Z.
  • Fig. S5. Growth and activity of stabilized lacZ plasmid in different oxygen, inducer (IPTG), and antibiotic conditions.
  • Fig. S6. CFU and stability of constructs in subcutaneous tumor models.
  • Fig. S7. Background cleavage of LuGal from urine assay.
  • Table S2. Results of three-way factorial ANOVA.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List and quantification of liver metastases.

[Download Table S1]