Supplementary Materials

Supplementary Material for:

Classic reaction kinetics can explain complex patterns of antibiotic action

Pia Abel zur Wiesch,* Sören Abel, Spyridon Gkotzis, Paolo Ocampo, Jan Engelstädter, Trevor Hinkley, Carsten Magnus, Matthew K. Waldor, Klas Udekwu, Ted Cohen

*Corresponding author. E-mail: pzw{at}daad-alumni.de

Published 13 May 2015, Sci. Transl. Med. 7, 287ra73 (2015)
DOI: 10.1126/scitranslmed.aaa8760

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Analysis of single-cell time-lapse microscopy data E. coli MG1655 cells exposed to tetracycline.
  • Fig. S2. Baseline growth rate affects length of post-antibiotic growth suppression.
  • Fig. S3. Time-kill curves of E. coli CAB1 exposed to five different classes of antibiotics in various concentrations.
  • Fig. S4. Analysis of single-cell time-lapse microscopy data for E. coli MG1655 cells exposed to streptomycin.
  • Fig. S5. Simulated time-kill curves under different assumptions.
  • Fig. S6. Distribution of drug susceptibility and expected time-kill curve.
  • Fig. S7. Effect of variance in target molecule content.
  • Fig. S8. Overview of kill mechanisms, chemical reactions, and compartmental models used in this study.
  • Table S1. Ciprofloxacin persisting E. coli have not acquired higher resistance to the antibiotic.
  • Table S2. Antibiotic concentrations used for experiments.
  • Table S3. Kinetic parameters for different antibiotics.
  • Table S4. Explanation of variables and parameters.
  • Legends for movies S1 and S2
  • References (6482)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Single-cell microscopy data for E. coli MG1655 cells exposed to tetracycline (12.5 mg/liter) (0.8× MIC).
  • Movie S2 (.avi format). Single-cell microscopy data for E. coli MG1655 cells exposed to streptomycin (25 mg/liter) (2× MIC).

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