Supplementary Materials

Supplementary Material for:

The nicotinic α6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors

Jeffrey S. Wieskopf, Jayanti Mathur, Walrati Limapichat, Michael R. Post, Mona Al-Qazzaz, Robert E. Sorge, Loren J. Martin, Dmitri V. Zaykin, Shad B. Smith, Kelen Freitas, Jean-Sebastien Austin, Feng Dai, Jie Zhang, Jaclyn Marcovitz, Alexander H. Tuttle, Peter M. Slepian, Sarah Clarke, Ryan M. Drenan, Jeff Janes, Shakir Al Sharari, Samantha K. Segall, Eske K. Aasvang, Weike Lai, Reinhard Bittner, Christopher I. Richards, Gary D. Slade, Henrik Kehlet, John Walker, Uwe Maskos, Jean-Pierre Changeux, Marshall Devor, William Maixner, Luda Diatchenko, Inna Belfer, Dennis A. Dougherty, Andrew I. Su, Sarah C. R. Lummis, M. Imad Damaj, Henry A. Lester, Ardem Patapoutian, Jeffrey S. Mogil*

*Corresponding author. E-mail: jeffrey.mogil{at}mcgill.ca

Published 13 May 2015, Sci. Transl. Med. 7, 287ra72 (2015)
DOI: 10.1126/scitranslmed.3009986

This PDF file includes:

  • Fig. S1. Hind paw withdrawal thresholds to von Frey fiber stimulation measured before (14, 7 days) and after (1, 4, 7, 14, 21, and 28 days) SNI surgery.
  • Fig. S2. Sex differences in Chrna6 DRG mRNA expression in SM mice and their correlation with sex differences in mechanical allodynia.
  • Fig. S3. Down-regulation of Chrna6 by nerve injury and correlation with mechanical allodynia.
  • Fig. S4. Increased ipsilateral mechanical allodynia in Chrna6 (α6 KO) but not Chrna4 null mutants (α4 KO).
  • Fig. S5. No altered sensitivity of Chrna6 KO mice in a battery of acute and tonic nociceptive assays.
  • Fig. S6. Physical contacts between P2X3 and α6β4* nAChRs revealed by FLIM.
  • Table S1. Affymetrix gene expression data of all Chrn* probes and correlation with baseline nociceptive sensitivity and mechanical allodynia.
  • Table S2. Top 10 correlated haplotypes (by P value), genome-wide, with SNI-induced mechanical allodynia in 25 mouse strains.
  • Table S3. Nicotine anti-allodynic ED50s in all genotypes.
  • Table S4. Dose-response characteristics (EC50 and Hill coefficients; mean ± SEM) for various combinations of α6-containing nicotinic receptors and P2X
    receptors expressed in oocytes.

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