Supplementary Materials

Supplementary Material for:

Identification of a plant isoflavonoid that causes biliary atresia

Kristin Lorent, Weilong Gong, Kyung A. Koo, Orith Waisbourd-Zinman, Sara Karjoo, Xiao Zhao, Ian Sealy, Ross N. Kettleborough, Derek L. Stemple, Peter A. Windsor, Stephen J. Whittaker, John R. Porter, Rebecca G. Wells,* Michael Pack*

*Corresponding author. E-mail: rgwells{at}mail.med.upenn.edu (R.G.W.); mpack{at}mail.med.upenn.edu (M.P.)

Published 6 May 2015, Sci. Transl. Med. 7, 286ra67 (2015)
DOI: 10.1126/scitranslmed.aaa1652

This PDF file includes:

  • Fig. S1. Swallowing function of biliatresone-treated larvae.
  • Fig. S2. Scheme for biliatresone isolation.
  • Fig. S3. Biliatresone-mediated biliary secretion defect.
  • Fig. S4. Biliatresone dose response in zebrafish.
  • Fig. S5. Biliatresone biliary secretion dose response.
  • Fig. S6. Macrophages not required for biliatresone toxicity.
  • Fig. S7. Biliatresone treatment during embryogenesis.
  • Fig. S8. No effect of biliatresone on digestive epithelia.
  • Fig. S9. Bile flow requirement for biliatresone toxicity.
  • Fig. S10. Zebrafish ductbend mutants.
  • Fig. S11. ductbend biliatresone sensitivity.
  • Fig. S12. ductbend candidate gene expression.
  • Fig. S13. Biliatresone-induced defects of cholangiocyte cilia and microtubules.
  • Fig. S14. Dose-dependent loss of cholangiocyte microtubules with biliatresone treatment.
  • Fig. S15. Effects of biliatresone on hepatocytes.
  • Fig. S16. Lack of effect of isoflavanone-3 on mammalian cholangiocyte spheroids.
  • Fig. S17. Effects of betavulgarin on mammalian cholangiocyte spheroids.
  • Table S1. Biliatresone toxicity in DAPT-treated larvae.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Biliary anatomy of a wild-type larva.
  • Movie S2 (.mov format). Biliary anatomy of a toxin-treated wild-type larva.

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