Supplementary Materials

Supplementary Material for:

Urinary metabolic signatures of human adiposity

Paul Elliott,* Joram M. Posma, Queenie Chan, Isabel Garcia-Perez, Anisha Wijeyesekera, Magda Bictash, Timothy M. D. Ebbels, Hirotsugu Ueshima, Liancheng Zhao, Linda van Horn, Martha Daviglus, Jeremiah Stamler, Elaine Holmes, Jeremy K. Nicholson*

*Corresponding author. E-mail: p.elliott{at}imperial.ac.uk (P.E.); j.nicholson{at}imperial.ac.uk (J.K.N.)

Published 29 April 2015, Sci. Transl. Med. 7, 285ra62 (2015)
DOI: 10.1126/scitranslmed.aaa5680

This PDF file includes:

  • Fig. S1. Distributions of the ICCs of the 1H NMR data.
  • Fig. S2. Scatterplot of observed BMI (UK) versus BMI estimated using UK data by the U.S. EN model (tables S13 and S14) (F test: F52,391 = 4.04, P = 9.12 × 10−16).
  • Table S1. Descriptive data of the INTERMAP study.
  • Table S2. Chemical shifts identified and identification strategy for each 1H NMR metabolite.
  • Table S3. ICC for each 1H NMR metabolite for the first and second urine collection data (obtained on average 3 weeks apart).
  • Table S4. Structurally unidentified 1H NMR–derived signals associated significantly with BMI in 1880 U.S. INTERMAP participants using first urine collection specimens.
  • Table S5. P value for a gender-interaction term included in the models for the U.S. INTERMAP population (n = 1880).
  • Table S6. P value for a gender-interaction term included in the models for the UK INTERMAP population (n = 444).
  • Table S7. Structurally unidentified 1H NMR–derived signals associated significantly with BMI in 1880 U.S. INTERMAP participants using second urine collection specimens.
  • Table S8. Structurally identified 1H NMR–derived urinary metabolites associated significantly with BMI in 1880 U.S. INTERMAP participants using second urine collection specimens.
  • Table S9. Association with BMI of metabolites identified by 1H NMR in 444 UK INTERMAP participants for second urine collection specimens.
  • Table S10. Full names and abbreviations of metabolites in the metabolic reaction network (Fig. 3).
  • Table S11. Abbreviations and closest affinity classifications of metabolites in the metabolic reaction network (Fig. 3).
  • Table S12. Metabolite-BMI associations adjusted for all other metabolites.
  • Table S13. EN model of all variables combined (λ = 0.15, α = 0.98).
  • Table S14. EN model of all variables combined (λ = 0.17, α = 0.59), excluding population sample variables.

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