Supplementary Materials

Supplementary Material for:

Calcium-sensing receptor antagonists abrogate airway hyperresponsiveness and inflammation in allergic asthma

Polina L. Yarova, Alecia L. Stewart, Venkatachalem Sathish, Rodney D. Britt Jr., Michael A. Thompson, Alexander P. P. Lowe, Michelle Freeman, Bharathi Aravamudan, Hirohito Kita, Sarah C. Brennan, Martin Schepelmann, Thomas Davies, Sun Yung, Zakky Cholisoh, Emma J. Kidd, William R. Ford, Kenneth J. Broadley, Katja Rietdorf, Wenhan Chang, Mohd E. Bin Khayat, Donald T. Ward, Christopher J. Corrigan, Jeremy P. T. Ward, Paul J. Kemp, Christina M. Pabelick, Y. S. Prakash,* Daniela Riccardi*

*Corresponding author. E-mail: riccardi{at}cf.ac.uk (D.R.); prakash.ys{at}mayo.edu (Y.S.P.);

Published 22 April 2015, Sci. Transl. Med. 7, 284ra60 (2015)
DOI: 10.1126/scitranslmed.aaa0282

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Negative controls and original Western blots for Fig. 1.
  • Fig. S2. Polycations increase [Ca2+]i by acting on the human CaSR.
  • Fig. S3. Calcilytics prevent CaSR activation in human asthmatic ASM.
  • Fig. S4. Technical replicates of data presented in Fig. 2E and summarized in Fig. 2F.
  • Fig. S5. Phenotypic characterization of the SM22aCaSRΔflox/Δflox mouse.
  • Fig. S6. Validation of the MA asthma model.
  • Fig. S7. CaSR expression in human eosinophils.
  • Reference (50)

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Other Supplementary Material for this manuscript includes the following:

  • Database S1. Source data for Figs. 1 to 5 and figs. S1 to S5 (provided as Excel file).

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