Supplementary Materials

Supplementary Material for:

Monoclonal antibodies against GARP/TGF-β1 complexes inhibit the immunosuppressive activity of human regulatory T cells in vivo

Julia Cuende, Stéphanie Liénart, Olivier Dedobbeleer, Bas van der Woning, Gitte De Boeck, Julie Stockis, Caroline Huygens, Didier Colau, Joan Somja, Philippe Delvenne, Muriel Hannon, Frédéric Baron, Laure Dumoutier, Jean-Christophe Renauld, Hans De Haard, Michael Saunders, Pierre G. Coulie, Sophie Lucas*

*Corresponding author. E-mail: sophie.lucas{at}uclouvain.be

Published 22 April 2015, Sci. Transl. Med. 7, 284ra56 (2015)
DOI: 10.1126/scitranslmed.aaa1983

This PDF file includes:

  • Fig. S1. MHG-8 and LHG-10 inhibit production of active TGF-β1 by human polyclonal Tregs.
  • Fig. S2. Some anti-hGARP mAbs bind free GARP only.
  • Fig. S3. Detailed results of experiments I, II, and III summarized in Fig. 5B.
  • Fig. S4. Analyses of tissues from NSG mice grafted with human PBMCs.
  • Fig. S5. FACS analysis of splenocytes collected from NSG mice at various time points after transfer of human PBMCs ± Tregs.
  • Fig. S6. Human fibroblasts express GARP but do not produce active TGF-β1.
  • Fig. S7. Gating strategies, isotype controls, and representative stains of all flow experiments.
  • Fig. S8. Source data for figures containing Western blot analyses.
  • Table S1. Purity of polyclonal human Tregs.

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