Supplementary Materials

Supplementary Material for:

Clinical impact of the NKp30/B7-H6 axis in high-risk neuroblastoma patients

Michaela Semeraro, Sylvie Rusakiewicz, Véronique Minard-Colin, Nicolas F. Delahaye, David Enot, Frédéric Vély, Aurélien Marabelle, Benjamin Papoular, Christelle Piperoglou, Mirco Ponzoni, Patrizia Perri, Andrei Tchirkov, Jessica Matta, Valérie Lapierre, Tala Shekarian, Sandrine Valsesia-Wittmann, Frédéric Commo, Nicole Prada, Vichnou Poirier-Colame, Brigitte Bressac, Sophie Cotteret, Laurence Brugieres, Françoise Farace, Nathalie Chaput, Guido Kroemer,* Dominique Valteau-Couanet, Laurence Zitvogel*

*Corresponding author. E-mail: laurence.zitvogel{at}gustaveroussy.fr (L.Z.); kroemer{at}orange.fr (G.K.)

Published 15 April 2015, Sci. Transl. Med. 7, 283ra55 (2015)
DOI: 10.1126/scitranslmed.aaa2327

This PDF file includes:

  • Fig. S1. Enumeration of various leukocyte subsets in PB and BM of localized or metastatic NB.
  • Fig. S2. Clinical relevance of the selective increase in CD3CD56bright NK cells in HR-NB.
  • Fig. S3. Survival of HR-NB according to NKp30 NK cell surface expression.
  • Fig. S4. Expression of NKp30 isoforms and prognosis in HR-NB.
  • Fig. S5. Stable expression of the NKp30 isoforms in HR-NB.
  • Fig. S6. sB7-H6 serum concentrations not correlated with the relative expression of NKp30 isoforms.
  • Fig. S7. Regulation of B7-H6 expression.
  • Fig. S8. Membrane expression of B7-H6 in M1- and M2-like polarized macrophages.
  • Fig. S9. Crosstalk between M1- or M2-like polarized macrophages and autologous NK cells.
  • Fig. S10. Direct proapoptotic effects of rTNFα and rIFNγ on various NB cell lines.
  • Fig. S11. Opposite roles of IL-10 and TNFα in the dialogue between monocytes, NB, and effector cells.
  • Fig. S12. Schematic representation of the interactions between NK, NB, and macrophages in the context of NKp30 isoform profiling.
  • Fig. S13. Restoration of NKp30 function by down-regulation of NKp30C with siRNA.
  • Table S1. Details of antibodies used for the immunophenotyping.
  • Table S2. Oligonucleotides used in this study.
  • Table S3. Exact P values.

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