Supplementary Materials

Supplementary Material for:

G-CSF mobilizes CD34+ regulatory monocytes that inhibit graft-versus-host disease

Maud D'Aveni, Julien Rossignol, Tereza Coman, Shivajanani Sivakumaran, Stephen Henderson, Teresa Manzo, Pedro Santos e Sousa, Julie Bruneau, Guillemette Fouquet, Flora Zavala, Olinda Alegria-Prévot, Meriem Garfa-Traoré, Felipe Suarez, Hélène Trebeden-Nègre, Mohamad Mohty, Clare L. Bennett, Ronjon Chakraverty, Olivier Hermine,* Marie-Thérèse Rubio*

*Corresponding author. E-mail: mt_rubio{at} (M.-T.R.); ohermine{at} (O.H.)

Published 1 April 2015, Sci. Transl. Med. 7, 281ra42 (2015)
DOI: 10.1126/scitranslmed.3010435

This PDF file includes:

  • Fig. S1. Human CD34+ monocytes inhibit allogeneic T cell proliferation in vitro and reduce weight loss in the NSG xenogeneic HSCT model.
  • Fig. S2. Posttransplant peripheral blood levels of proliferating CD4+ T cells and Tregs in patients who developed grade II to IV acute GVHD and those who did not.
  • Fig. S3. The transcriptional profile of iNOS−/− CD34+ monocytes is very similar to their wild-type counterparts.
  • Fig. S4. CD34+ monocytes patrolling in the lymph nodes and spleen 15 days after in vivo injection have kept the same phenotype and function as input cells.
  • Fig. S5. Apoptotic T cells are engulfed by splenic macrophages in allo-SCT recipient mice that received CD34+ monocytes.
  • Fig. S6. Mice receiving allo-SCT with CD34+ monocytes have increased serum TGF-β1 levels.
  • Fig. S7. Human CD34+ monocytes inhibit allogeneic T cell proliferation in an MLR through iNOS.
  • Fig. S8. The functions and phenotype of ex vivo expanded CD34+ cells are similar to that of freshly sorted cells.

[Download PDF]