Supplementary Materials

Supplementary Material for:

A microRNA-Hippo pathway that promotes cardiomyocyte proliferation and cardiac regeneration in mice

Ying Tian,* Ying Liu, Tao Wang, Ning Zhou, Jun Kong, Li Chen, Melinda Snitow, Michael Morley, Deqiang Li, Nataliya Petrenko, Su Zhou, Minmin Lu, Erhe Gao, Walter J. Koch, Kathleen M. Stewart, Edward E. Morrisey*

*Corresponding author. E-mail: ying.tian{at}temple.edu (Y.T.); emorrise{at}mail.med.upenn.edu (E.E.M.)

Published 18 March 2015, Sci. Transl. Med. 7, 279ra38 (2015)
DOI: 10.1126/scitranslmed.3010841

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Generation of mice with a conditional deletion of the miR302-367 cluster.
  • Fig. S2. Generation of the mice with conditional overexpression of the miR302-367 cluster.
  • Fig. S3. miR302-367 regulates cardiomyocyte proliferation through the Hippo pathway.
  • Fig. S4. Gene expression profiles in adult hearts after inducible overexpression of miR302-367.
  • Fig. S5. Hippo signaling activity and myocardial features in the adult heart after inducible overexpression of miR302-367.
  • Fig. S6. Half-life of miR302-367 mimic treatment and the effects on cardiomyocyte proliferation, apoptosis, and vascular perfusion.
  • Fig. S7. Expression of miR302 mimics in the lung and organ histology after systemic treatment with mimics.
  • Table S1. miRNAs identified from HITS-CLIP.
  • Table S2. Overlapping genes between HITS-CLIP and predicted targets of miR302.
  • Table S3. miR302 targets identified from HITS-CLIP.
  • Table S4. miRNA qRT-PCR analysis in the adult mouse heart.
  • Table S5. Primers for genotyping, qRT-PCR, and luciferase reporter analyses.
  • References (41, 42)

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