Supplementary Materials

Supplementary Material for:

MG53-mediated cell membrane repair protects against acute kidney injury

Pu Duann, Haichang Li, Peihui Lin, Tao Tan, Zhen Wang, Ken Chen, Xinyu Zhou, Kristyn Gumpper, Hua Zhu, Thomas Ludwig, Peter J. Mohler, Brad Rovin, William T. Abraham, Chunyu Zeng, Jianjie Ma*

*Corresponding author. E-mail: jianjie.ma{at}osumc.edu

Published 18 March 2015, Sci. Transl. Med. 7, 279ra36 (2015)
DOI: 10.1126/scitranslmed.3010755

This PDF file includes:

  • Fig. S1. Ultrastructural examination of kidneys from wild-type and Mg53−/− mice.
  • Fig. S2. Pha-E lectin staining of primary cultured PTE cells.
  • Fig. S3. Recapitulation of MG53-mediated cell membrane repair in human renal proximal tubular cells.
  • Fig. S4. Abnormal membrane structures in Mg53−/− PTE cells.
  • Fig. S5. rhMG53 concentration in the PTE compartment of I/R-injured rat kidney.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Original data and P values (provided as a separate Excel file).
  • Movie S1 (.avi format). GFP-MG53 translocation to injury sites on human HKC-8 cells.
  • Movie S2 (.avi format). GFP-C242A expressed in HKC-8 cells not responding to acute membrane injury.
  • Movie S3 (.avi format). Microelectrode penetration into PTE cells derived from wild-type mice.
  • Movie S4 (.avi format). Microelectrode penetration into PTE cells derived from Mg53−/− mice.

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