Supplementary Materials

Supplementary Material for:

Immunological mechanisms of the antitumor effects of supplemental oxygenation

Stephen M. Hatfield, Jorgen Kjaergaard, Dmitriy Lukashev, Taylor H. Schreiber, Bryan Belikoff, Robert Abbott, Shalini Sethumadhavan, Phaethon Philbrook, Kami Ko, Ryan Cannici, Molly Thayer, Scott Rodig, Jeffrey L. Kutok, Edwin K. Jackson, Barry Karger, Eckhard R. Podack, Akio Ohta, Michail V. Sitkovsky*

*Corresponding author. E-mail:

Published 4 March 2015, Sci. Transl. Med. 7, 277ra30 (2015)
DOI: 10.1126/scitranslmed.aaa1260

This PDF file includes:

  • Materials and Methods
    Fig. S1. The tumor-regressing effects of respiratory hyperoxia are lost in cγ/Rag-2−/− mice.
  • Fig. S2. ROS scavenger does not prevent the antitumor effects of respiratory hyperoxia.
  • Fig. S3. Respiratory hyperoxia reverses hypoxia-adenosinergic inhibition of NK cells.
  • Fig. S4. Respiratory hyperoxia does not further improve the activity of tumor-reactive A2AR−/− T cells.
  • Fig. S5. CD8 and CD4 T cells avoid hypoxic TME.
  • Fig. S6. Tregs with higher expression of CTLA-4 are more hypoxic.
  • Fig. S7. CD8 T cells from TDLN are enriched after culture activation for adoptive transfer.
  • Fig. S8. Breathing 60% oxygen increased IFN-γ production by CD8 T cells in the lung TME.
  • Table S1. Immunostimulating cytokines/chemokines increased by respiratory hyperoxia.
  • Table S2. Full list of primer sets in RT-PCR arrays.

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