Supplementary Materials

Supplementary Material for:

Functional characterization of IgA-targeted bacterial taxa from undernourished Malawian children that produce diet-dependent enteropathy

Andrew L. Kau, Joseph D. Planer, Jie Liu, Sindhuja Rao, Tanya Yatsunenko, Indi Trehan, Mark J. Manary, Ta-Chiang Liu, Thaddeus S. Stappenbeck, Kenneth M. Maleta, Per Ashorn, Kathryn G. Dewey, Eric R. Houpt, Chyi-Song Hsieh, Jeffrey I. Gordon*

*Corresponding author. E-mail: jgordon@wustl.edu

Published 25 February 2015, Sci. Transl. Med. 7, 276ra24 (2015)
DOI: 10.1126/scitranslmed.aaa4877

This PDF file includes:

  • Fig. S1. Characterization of gnotobiotic mice receiving intact uncultured fecal microbiota from discordant twins and the experimental design for subsequent
    transplantation of IgA+ consortia purified from their fecal microbiota into germ-free recipients.
  • Fig. S2. Validation of BugFACS using an artificial two-member bacterial community.
  • Fig. S3. Control experiments validating the specificity of BugFACS.
  • Fig. S4. Combining HMIgA+ and KMIgA+ consortia attenuates early weight loss in recipient gnotobiotic animals.
  • Fig. S5. Serum cytokine profiles in KMIgA+ animals and a comparison of immunophenotypes in recipients of KMIgA+, HMIgA+, and MixIgA+ consortia.
  • Fig. S6. KMF2IgA+ microbiota transmits a colonic epithelial discohesion phenotype to recipient gnotobiotic mice.
  • Fig. S7. IgA-targeted bacterial taxa in the kwashiorkor microbiota disrupt the small intestinal epithelial barrier in recipient gnotobiotic mice.
  • Fig. S8. Representation of bacterial taxa within the gut microbiota of gnotobiotic mice colonized with IgA+ consortia.
  • Fig. S9. Heatmap of bacterial family-level taxa represented in the fecal microbiota of gnotobiotic mice sampled 12 days after receiving IgA+ consortia from
    discordant twin pair 46 or 80.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Clinical characteristics of Malawian twin microbiota donors.
  • Table S2 (Microsoft Excel format). IgA indices determined from BugFACS analysis of fecal samples obtained from mice colonized with the fecal microbiota
  • of co-twins discordant for kwashiorkor (pair 57).
  • Table S3 (Microsoft Excel format). Characteristics of the genome sequences and content of virulence factors of Enterobacteriaceae isolates obtained from in vitro culture of IgA+ consortia or from spleens of mice colonized with KMF2IgA+ cecal microbiota (originating from donor 57A).
  • Table S4 (Microsoft Excel format). IgA indices and relative abundances of family- and genus-level bacterial taxa in two cohorts of Malawian infants.
  • Table S5 (Microsoft Excel format). Clinical characteristics of individuals from the LCNI-5.
  • Table S6 (Microsoft Excel format). Multiplex quantitative PCR analysis of enteropathogen burden in members of the LCNI-5 cohort.
  • Table S7 (Microsoft Excel format). Subgroup analysis of LCNI-5 cohort.

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