Supplementary Materials

Supplementary Material for:

Regulation of immune responses to protein therapeutics by transplacental induction of T cell tolerance

Nimesh Gupta, Slobodan Culina, Yann Meslier, Jordan Dimitrov, Christophe Arnoult, Sandrine Delignat, Bagirath Gangadharan, Maxime Lecerf, Sune Justesen, Valérie Gouilleux-Gruart, Benoit L. Salomon, David W. Scott, Srinivas V. Kaveri, Roberto Mallone, Sébastien Lacroix-Desmazes*

*Corresponding author. E-mail: sebastien.lacroix-desmazes{at}

Published 18 February 2015, Sci. Transl. Med. 7, 275ra21 (2015)
DOI: 10.1126/scitranslmed.aaa1957

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Schematic representation of Fc-fused constructs.
  • Fig. S2. Validation of Fc-fused proteins.
  • Fig. S3. Transcytosis of HA1.
  • Fig. S4. Transplacental transfer of HA1Fc.
  • Fig. S5. Transplacentally transferred HA1Fc reaching a fetal lymphoid organ.
  • Fig. S6. Optimal time window for induction of Ag-specific Tregs.
  • Fig. S7. Detection of transplacentally transferred HA1Fc in fetal thymic epithelial cells.
  • Fig. S8. Transplacental transfer of C2Fc.
  • Fig. S9. Transplacentally transferred C2Fc reaching a fetal lymphoid organ.
  • Fig. S10. Gating strategy used to identify cell subsets taking up HA1Fc in fetal spleen and thymus.
  • Table S1. Sequence of the primers used for cloning Fcγ1-fused chimeric proteins.

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