Supplementary Materials

Supplementary Material for:

Effect of selective LRRK2 kinase inhibition on nonhuman primate lung

Reina N. Fuji,* Michael Flagella, Miriam Baca, Marco A. S. Baptista, Jens Brodbeck, Bryan K. Chan, Brian K. Fiske, Lee Honigberg, Adrian M. Jubb, Paula Katavolos, Donna W. Lee, Sock-Cheng Lewin-Koh, Tori Lin, Xingrong Liu, Shannon Liu, Joseph P. Lyssikatos, Jennifer O'Mahony, Mike Reichelt, Merone Roose-Girma, Zejuan Sheng, Todd Sherer, Ashley Smith, Margaret Solon, Zachary K. Sweeney, Jacqueline Tarrant, Alison Urkowitz, Søren Warming, Murat Yaylaoglu, Shuo Zhang, Haitao Zhu, Anthony A. Estrada, Ryan J. Watts*

*Corresponding author. E-mail: fuji.reina{at} (R.N.F.); watts.ryan{at} (R.J.W.)

Published 4 February 2015, Sci. Transl. Med. 7, 273ra15 (2015)
DOI: 10.1126/scitranslmed.aaa3634

This PDF file includes:

  • Materials and Methods
  • Fig. S1. LRRK2 mRNA expression in human tissues.
  • Fig. S2. LRRK2 mRNA expression in selected WT mouse tissues.
  • Fig. S3. Lung phenotype of Lrrk2 KO mice.
  • Fig. S4. Kidney phenotype of Lrrk2 KO mice.
  • Fig. S5. Western blots of mouse pharmacodynamic data.
  • Fig. S6. Plasma pharmacokinetic and brain and CSF data of 7915 in NHPs from 7-day study.
  • Fig. S7. Western blots of NHP brain pharmacodynamic data from 7-day study.
  • Fig. S8. Plasma pharmacokinetic and brain and CSF data of 0877 and 7915 in NHPs from 29-day study.
  • Fig. S9. Effect of 7915 on NHP monocytes.
  • Fig. S10. Western blots of NHP pharmacodynamic data from 29-day study.
  • Fig. S11. Effect of 0877 and 7915 on NHP kidney.
  • Fig. S12. Di-22:6-BMP in NHP CSF.
  • Fig. S13. Diagram of gene targeting strategy for Lrrk2 EGFP KI mice (Lrrk2 KO).
  • Table S1. Kinase profile data from Invitrogen at 1 mM.
  • Table S2. TK data of 7915 and 0877 in mice.
  • Table S3. TK data of 7915 and 0877 in rats.
  • Table S4. Monkey surfactant protein D ELISA results.
  • Table S5. Results for surfactant protein A (SpA) and surfactant protein D (SpD) in mouse serum.
  • Reference (38)

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