Supplementary Materials

Supplementary Material for:

Dual suppression of estrogenic and inflammatory activities for targeting of endometriosis

Yuechao Zhao, Ping Gong, Yiru Chen, Jerome C. Nwachukwu, Sathish Srinivasan, CheMyong Ko, Milan K. Bagchi, Robert N. Taylor, Kenneth S. Korach, Kendall W. Nettles, John A. Katzenellenbogen, Benita S. Katzenellenbogen*

*Corresponding author. E-mail: katzenel{at}illinois.edu

Published 21 January 2015, Sci. Transl. Med. 7, 271ra9 (2015)
DOI: 10.1126/scitranslmed.3010626

This PDF file includes:

  • Fig. S1. OBHS and CLI levels in serum over 2 weeks after pellet implantation.
  • Fig. S2. Effect of OBHS or CLI on endometriotic lesion establishment and eutopic uterine weight.
  • Fig. S3. Antiestrogenic effects of ligands on eutopic uterine tissues of ovariectomized mice treated with E2.
  • Fig. S4. Effects of OBHS and CLI on eutopic uteri.
  • Fig. S5. Effects of OBHS and CLI on mammary gland.
  • Fig. S6. Effects of OBHS and CLI on ovary and estrous cycles.
  • Fig. S7. Suppression by OBHS and CLI of immune cell infiltration into ectopic lesions.
  • Fig. S8. Effects of ligands on neovascularization and innervation in ectopic lesions.
  • Fig. S9. Expression of Cyp19a1 (aromatase) mRNA in ectopic lesions.
  • Fig. S10. Effect of ligands on crosstalk between endometriotic and immune cells.
  • Fig. S11. Reduction of infiltrating M1 and M2 macrophages in ectopic lesions by OBHS or CLI.
  • Fig. S12. Effect of macrophage depletion on preventive effects of ligands.
  • Fig. S13. Uterine weights of wild-type or ER transgenic mice after ligand treatment.
  • Table S1. Fertility after treatment with OBHS or CLI.
  • Table S2. List of primary antibodies used in these studies.
  • Table S3. Primer sets used for quantitative PCR analysis.
  • Legends for tables S4 and S5

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Other Supplementary Material for this manuscript includes the following:

  • Table S4. Original data for individual animals (provided as a separate Excel file).
  • Table S5. Statistics (P values) for data (provided as a separate Excel file).

[Download Tables S4 and S5]