Supplementary Materials

Supplementary Material for:

A Wnt-TGFβ2 axis induces a fibrogenic program in muscle stem cells from dystrophic mice

Stefano Biressi, Elen H. Miyabara, Suchitra D. Gopinath, Poppy M. M. Carlig, Thomas A. Rando*

*Corresponding author. E-mail: rando{at}stanford.edu

Published 17 December 2014, Sci. Transl. Med. 6, 267ra176 (2014)
DOI: 10.1126/scitranslmed.3008411

This PDF file includes:

  • Methods
  • Fig. S1. Fibrotic features are induced in satellite cells in mdx5Cv mice.
  • Fig. S2. Cells of the satellite cell lineage are present in the muscle interstitium in mdx5Cv mice.
  • Fig. S3. In vitro alteration of canonical Wnt signaling.
  • Fig. S4. β-Catenin binds to the Tgfβ2 locus.
  • Fig. S5. Expression of TGFβ receptors in satellite cell progeny.
  • Fig. S6. Wnt-TGFβ2 axis induces fibrogenic features in myoblasts in vitro.
  • Fig. S7. The Wnt-Tgfβ2 axis is active in dystrophic muscles.
  • Fig. S8. TGFβ signaling induces fibrogenic features in satellite cells in mdx5Cv muscles.
  • Fig. S9. Blocking TGFβ signaling ameliorates the dystrophic phenotype.
  • Table S1. List of genes that are significantly increased (≥2.5-fold) in Wnt3a-treated myoblasts.
  • Table S2. Primer list for genotyping PCR.
  • Table S3. Primer list for SYBR Green real-time PCR.
  • Table S4. Antibody list for immunofluorescence.
  • Table S5. Primer list for ChIP assay.
  • References (9194)

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