Supplementary Material for:

A rapid antimicrobial susceptibility test based on single-cell morphological analysis

Jungil Choi, Jungheon Yoo, Mincheol Lee, Eun-Geun Kim, Ji Soo Lee, Seungok Lee, Seik Joo, Sang Hoon Song, Eui-Chong Kim, Jung Chan Lee, Hee Chan Kim, Yong-Gyun Jung,* Sunghoon Kwon*

*Corresponding author. E-mail: skwon{at}snu.ac.kr (S.K.); jygpaul{at}quantamatrix.com (Y.-G.J.)

Published 17 December 2014, Sci. Transl. Med. 6, 267ra174 (2014)
DOI: 10.1126/scitranslmed.3009650

This PDF file includes:

• Materials and Methods
• Fig. S1. MAC chip.
• Fig. S2. Diffusion characteristics of Rhodamine B in the imaging area of the MAC chip.
• Fig. S3. Time-lapse images of bacteria at the different locations in the MAC chip.
• Fig. S4. MIC determination for E. coli ATCC 25922 by analyzing bacterial number and size in response to antibiotics.
• Fig. S5. MIC determination for S. aureus ATCC 29213 by analyzing bacterial number in response to antibiotics.
• Fig. S6. MIC determination for E. faecalis ATCC 29212 by analyzing bacterial number in response to antibiotics.
• Fig. S7. Automated image processing for representative Gram-positive strains.
• Fig. S8. Automated image processing for representative Gram-negative strains.
• Table S1. Diffusion times into the imaging region.
• Table S2. Molecular weight of the antimicrobial agents tested in the clinical AST.
• Table S3. Morphological characteristics of E. coli ATCC 25922 in response to different antimicrobial agents at concentration equal to or higher than the MIC.
• Table S4. Morphological characteristics of P. aeruginosa ATCC 27853 for different antimicrobial agents.
• Table S5. Morphological characteristics of S. aureus ATCC 29213 for different antimicrobial agents.
• Table S6. Morphological characteristics of E. faecalis ATCC 29212 for different antimicrobial agents.
• Table S7. Explanation of image-processing algorithm.
• Legends for movies S1 and S2

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.wmv format). The Gram-negative P. aeruginosa ATCC 27853 divided at concentrations lower than the MIC of aztreonam.
• Movie S2 (.wmv format). The Gram-negative P. aeruginosa ATCC 27853 formed filaments at concentrations of β-lactam antimicrobial greater than the MIC.
• Supplementary file [SummaryDataAST.xlsx]. Summary of antimicrobial susceptibility test results from SCMA and the commercial automated systems
  (VITEK 2 and MicroScan) using clinical strains of SNUH and ISMH.

[Download Movies S1 to S2]

[Download Supplementary File]