Supplementary Materials

Supplementary Material for:

Semen enhances HIV infectivity and impairs the antiviral efficacy of microbicides

Onofrio Zirafi, Kyeong-Ae Kim, Nadia R. Roan, Silvia F. Kluge, Janis A. Müller, Shibo Jiang, Benjamin Mayer, Warner C. Greene, Frank Kirchhoff, Jan Münch*

*Corresponding author. E-mail: jan.muench{at}uni-ulm.de

Published 12 November 2014, Sci. Transl. Med. 6, 262ra157 (2014)
DOI: 10.1126/scitranslmed.3009634

This PDF file includes:

  • Fig. S1. Decreased antiviral efficacy of SPL7013 against semen-treated transmitted/founder HIV variants.
  • Fig. S2. Polyanions lose antiviral efficacy in the presence of semen and are cytotoxic at elevated concentrations.
  • Fig. S3. Decreased antiviral activity of the 2G12 antibody against semen-treated HIV and lack of cytotoxicity of nonpolyanionic compounds.
  • Fig. S4. Decreased antiviral activity of candidate microbicides against SEVI exposed R5-tropic HIV.
  • Fig. S5. Semen from patients with ejaculatory duct obstruction that lacks amyloid and viral-enhancing activity does not antagonize the antiviral activity of a polyanionic microbicide.
  • Fig. S6. Schematic of in vitro assay and results.

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