Supplementary Materials

Supplementary Material for:

Endoscopic molecular imaging of human bladder cancer using a CD47 antibody

Ying Pan, Jens-Peter Volkmer, Kathleen E. Mach, Robert V. Rouse, Jen-Jane Liu, Debashis Sahoo, Timothy C. Chang, Thomas J. Metzner, Lei Kang, Matt van de Rijn, Eila C. Skinner, Sanjiv S. Gambhir, Irving L. Weissman, Joseph C. Liao*

*Corresponding author. E-mail: jliao{at}stanford.edu

Published 29 October 2014, Sci. Transl. Med. 6, 260ra148 (2014)
DOI: 10.1126/scitranslmed.3009457

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Software algorithm for fluorescence intensity analysis of confocal videos.
  • Fig. S2. Fluorescence signal reproducibility of CD47 imaging using confocal endomicroscopy.
  • Fig. S3. Colocalization of blue light cystoscopy images and histology by intravesical dye injection.
  • Fig. S4. Immunofluorescence detection of tissue-bound anti-CD47 after imaging.
  • Table S1. Patient characteristics and histopathological diagnoses.
  • Table S2. Fluorescence intensity of anti-CD47 binding of bladder cancer and adjacent normal bladder mucosa using confocal endomicroscopy.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Ex vivo endoscopic molecular imaging of papillary urothelial carcinoma using anti-CD47.
  • Movie S2 (.mov format). Ex vivo endoscopic molecular imaging of bladder CIS using anti-CD47.
  • Movie S3 (.mov format). Ex vivo endoscopic molecular imaging of bladder inflammation using anti-CD47.
  • Movie S4 (.mov format). Ex vivo endoscopic molecular imaging of recurrent carcinoma after BCG treatment using anti-CD47.

[Download Movies S1 to S4]