Supplementary Materials

Supplementary Material for:

TOPK inhibitor induces complete tumor regression in xenograft models of human cancer through inhibition of cytokinesis

Yo Matsuo, Jae-Hyun Park, Takashi Miyamoto, Shinji Yamamoto, Shoji Hisada, Houda Alachkar, Yusuke Nakamura*

*Corresponding author. E-mail: ynakamura{at}

Published 22 October 2014, Sci. Transl. Med. 6, 259ra145 (2014)
DOI: 10.1126/scitranslmed.3010277

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Correlation between TOPK expression and clinical outcome.
  • Fig. S2. Cell-based antiproliferative assay for OTS514.
  • Fig. S3. LU-99 lung cancer xenograft model treated with intravenous administration of free OTS514.
  • Fig. S4. Cell-based antiproliferative assay for OTS964.
  • Fig. S5. Expression of Src family kinases in cancer cell lines.
  • Fig. S6. Inhibition of TOPK activity by OTS964 treatment.
  • Fig. S7. Enhancement of HSC differentiation to megakaryocytes by TOPK inhibitors.
  • Fig. S8. Histological examination of liver toxicity with liposomal OTS964.
  • Fig. S9. Cytokinetic defects in LU-99 cells after administration of liposomal OTS964.
  • Fig. S10. Effects of liposomal OTS964 on proliferation and death of cancer cells.
  • Fig. S11. Morphological changes and apoptosis of cancer cells after treatment with liposomal OTS964.
  • Fig. S12. LU-99 xenograft model treated by oral administration of free OTS964.
  • Fig. S13. Immunohistochemical staining of LU-99 tumor sections after oral administration of free OTS964.
  • Table S1. Kinase profile analysis of OTS514 and OTS964.
  • Legends for videos S1 and S2
  • References (3741)

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Other Supplementary Material for this manuscript includes the following:

  • Table S2. Original data for composite figures from in vitro and in vivo experiments including P values (provided as a separate Excel file).
  • Video S1 (.mov format). Cytokinetic defect and apoptosis of T47D cells caused by OTS964 treatment.
  • Video S2 (.mov format). Normal proliferation of T47D cells.

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[Download Videos S1 and S2]