Supplementary Materials

Supplementary Material for:

EbpA vaccine antibodies block binding of Enterococcus faecalis to fibrinogen to prevent catheter-associated bladder infection in mice

Ana L. Flores-Mireles, Jerome S. Pinkner, Michael G. Caparon,* Scott J. Hultgren*

*Corresponding author. E-mail: hultgren{at}borcim.wustl.edu (S.J.H.); caparon{at}borcim.wustl.edu (M.G.C.)

Published 17 September 2014, Sci. Transl. Med. 6, 254ra127 (2014)
DOI: 10.1126/scitranslmed.3009384

This PDF file includes:

  • Fig. S1. E. faecalis OG1RF growth and biofilm formation in urine.
  • Fig. S2. Purified Ebp subunits.
  • Fig. S3. Reactivity of polyclonal mouse anti-EbpA antibodies against EbpA variant proteins.
  • Fig. S4. E. faecalis replication in bladder (A) and catheter (B) in a murine model of CAUTIs.
  • Fig. S5. EbpAFull immunization induces long-lasting, high-titer antibody responses.
  • Fig. S6. Murine serum titers to Ebp subunits and EbpA domains.
  • Fig. S7. Model of E. faecalis pathogenesis during CAUTIs.
  • Table S1. Strains used in this study.
  • Table S2. Plasmids used in this study.
  • Table S3. Primers used in this study.
  • References (52–54)

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