Supplementary Materials

Supplementary Material for:

Tumor clone dynamics in lethal prostate cancer

Suzanne Carreira, Alessandro Romanel, Jane Goodall, Emily Grist, Roberta Ferraldeschi, Susana Miranda, Davide Prandi, David Lorente, Jean-Sebastien Frenel, Carmel Pezaro, Aurelius Omlin, Daniel Nava Rodrigues, Penelope Flohr, Nina Tunariu, Johann S. de Bono, Francesca Demichelis,* Gerhardt Attard*

*Corresponding author. E-mail: gerhardt.attard{at} (G.A.); demichelis{at} (F.D.)

Published 17 September 2014, Sci. Transl. Med. 6, 254ra125 (2014)
DOI: 10.1126/scitranslmed.3009448

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Precision and false discovery rate (FDR) in silico estimations for point mutation (PM) at different allelic fractions.
  • Fig. S2. Sensitivity and specificity in silico estimations for monoallelic deletion and a range of tumor content values.
  • Fig. S3. AR and TP53 point mutation validation by digital PCR.
  • Fig. S4. TP53 copy number assessment by digital PCR.
  • Fig. S5. Allelic fraction patterns of informative SNPs within 21q22, 8p21, and 10q23 genomic regions for each sample and patient.
  • Fig. S6. Western blot of transfected AR wild type and four different clones of AR-L702H (C1 to C4) in PC-3 cells.

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). SNP mutation allele frequencies.
  • Table S2 (Microsoft Excel format). Patient characteristics.
  • Table S3 (Microsoft Excel format). Samples sequenced.
  • Table S4 (Microsoft Excel format). Coverage statistics summary.
  • Table S5 (Microsoft Excel format). Somatic point mutation data.
  • Table S6 (Microsoft Excel format). SPOP aberration frequencies in previous studies.
  • Table S7 (Microsoft Excel format). Tumor content data.
  • Table S8 (Microsoft Excel format). Dominance analysis detailed data.
  • Table S9 (Microsoft Excel format). Validation of sequencing data by digital droplet PCR.
  • Table S10 (Microsoft Excel format). Copy number data for main regions and AR gene.
  • Table S11 (Microsoft Excel format). Amplicon coordinates.
  • Table S12 (Microsoft Excel format). Germ-line sample sequencing statistics.

[Download Tables S1 to S12]