Supplementary Materials

Supplementary Material for:

Denervation suppresses gastric tumorigenesis

Chun-Mei Zhao, Yoku Hayakawa, Yosuke Kodama, Sureshkumar Muthupalani, Christoph B. Westphalen, Gøran T. Andersen, Arnar Flatberg, Helene Johannessen, Richard A. Friedman, Bernhard W. Renz, Arne K. Sandvik, Vidar Beisvag, Hiroyuki Tomita, Akira Hara, Michael Quante, Zhishan Li, Michael D. Gershon, Kazuhiro Kaneko, James G. Fox, Timothy C. Wang,* Duan Chen*

*Corresponding author. E-mail: duan.chen@ntnu.no (D.C.); tcw21@columbia.edu (T.C.W.)

Published 20 August 2014, Sci. Transl. Med. 6, 250ra115 (2014)
DOI: 10.1126/scitranslmed.3009569

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Flowchart showing the animal study design.
  • Fig. S2. Anterior UVT in mice.
  • Fig. S3. Body weight of male and female INS-GAS mice after surgery.
  • Fig. S4. Thickness of the gastric oxyntic mucosa after surgery in INS-GAS mice.
  • Fig. S5. Pathological scores for the stomach after surgery in INS-GAS mice.
  • Fig. S6. Pathological scores for the stomach after Botox injection in INS-GAS mice.
  • Fig. S7. Wnt signaling in INS-GAS mice compared with wild-type mice.
  • Fig. S8. Altered signaling pathways after vagotomy in INS-GAS mice.
  • Fig. S9. Wnt and Notch signaling pathways after vagotomy in INS-GAS mice.
  • Fig. S10. Immunostaining of CD44 after vagotomy in INS-GAS mice.
  • Fig. S11. Numbers of CD44-immunoreactive cells after Botox treatment ± vagotomy in INS-GAS mice.
  • Fig. S12. Tumor regeneration in the stomach after vagotomy in INS-GAS mice.
  • Fig. S13. Wnt signaling KEGG pathway in M3KO mice compared with wild-type mice.
  • Fig. S14. Altered signaling pathways in human gastric cancer tissue compared with adjacent noncancerous tissue.
  • Fig. S15. Gastric stump cancer after distal gastrectomy with or without vagotomy.
  • Fig. S16. Effect of 5-FU and oxaliplatin in INS-GAS mice.
  • Table S1. Animal experimental groups.
  • Table S2. Cohorts of gastric cancer patients.
  • Table S3. List of qRT-PCR primers used in this study.

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