Supplementary Materials

Supplementary Material for:

Deficient human β-defensin 1 underlies male infertility associated with poor sperm motility and genital tract infection

Ruiying Diao, Kin Lam Fok, Hao Chen, Mei Kuen Yu, Yonggang Duan, Chin Man Chung, Zhao Li, Hanwei Wu, Zesong Li, Hu Zhang, Ziliang Ji, Wanhua Zhen, Chi Fai Ng, Yaoting Gui, Zhiming Cai,* Hsiao Chang Chan*

*Corresponding author. E-mail: hsiaocchan@cuhk.edu.hk (H.C.C.); caizhiming2000@163.com (Z.C.)

Published 13 August 2014, Sci. Transl. Med. 6, 249ra108 (2014)
DOI: 10.1126/scitranslmed.3009071

This PDF file includes:

  • Materials and Methods
    Fig. S1. Decreased expression of DEFB1 in sperm from asthenozoospermia and leukocytospermia patients.
  • Fig. S2. Specificity of anti-DEFB1 antibody.
  • Fig. S3. Correlation between DEFB1 expression and sperm motility in normal and asthenozoospermia sperm.
  • Fig. S4. Inverse correlation between DEFB1 expression and number of leukocytes in normal and leukocytospermia sperm.
  • Fig. S5. Inverse correlation between sperm motility and number of leukocytes in normal and leukocytospermia sperm.
  • Fig. S6. Transfer of sperm-bound DEFB1 to bacteria after incubation with sperm.
  • Fig. S7. Negative effect of DEFB-neutralizing antibodies on sperm agglutination.
  • Fig. S8. Reversal of anti-DEFB1 antibody effect by rDEFB1.
  • Fig. S9. Negative effect of normal IgG on bactericidal activity of sperm.
  • Fig. S10. Effect of immunodepletion of DEFB1 by a separate source of DEFB1 antibody on sperm motility and bactericidal activity.
  • Fig. S11. Comparison of expression of sperm-bound and recombinant DEFB1.
  • Fig. S12. Negative effect of rDEFB1 on acrosome reaction.
  • Fig. S13. Effect of immunodepletion of DEFB1 on hyperactivated motility.
  • Fig. S14. Counteracting the reversal effect of DEFB1 antibody on rDEFB1 treatment by addition of preabsorbed rDEFB1.
  • Fig. S15. Expression of CCR6 receptor in human and mouse sperm.
  • Fig. S16. Effect of CCR6 ligand CCL20 on intracellular Ca2+ concentrations in normal sperm.
  • Fig. S17. Effect of rDEFB1 on cAMP production in normal and abnormal sperm.
  • Fig. S18. Expression of DEFB1 in human epididymis.
  • Fig. S19. Correlation between expression of DEFB1 and CCR6 in normal and infertile sperm.
  • Fig. S20. Negative effect of neutralizing antibodies against CCR6 on sperm agglutination.
  • Fig. S21. Effect of rDEFB1 and anti-CCR6 antibody on bactericidal activity in the absence of sperm.
  • Table S1. Clinical parameters of normal, asthenozoospermia, and leukocytospermia patients’ sperm used in DEFB1 expression profiling.
  • Table S2. Infection and fertility status of leukocytospermia patients used in DEFB1 expression profiling.
  • References (5156)

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Other Supplementary Material for this manuscript includes the following:

  • Table S3. Original data (provided as an Excel file).

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