Supplementary Materials

Supplementary Material for:

B cells populating the multiple sclerosis brain mature in the draining cervical lymph nodes

Joel N. H. Stern, Gur Yaari, Jason A. Vander Heiden, George Church, William F. Donahue, Rogier Q. Hintzen, Anita J. Huttner, Jon D. Laman, Rashed M. Nagra, Alyssa Nylander, David Pitt, Sriram Ramanan, Bilal A. Siddiqui, Francois Vigneault, Steven H. Kleinstein,* David A. Hafler,* Kevin C. O�Connor*

*Corresponding author. E-mail: steven.kleinstein@yale.edu (S.H.K.); david.hafler@yale.edu (D.A.H.); kevin.oconnor@yale.edu (K.C.O.)

Published 6 August 2014, Sci. Transl. Med. 6, 248ra107 (2014)
DOI: 10.1126/scitranslmed.3008879

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Distribution for the expected frequency of different types of edges in the lineage trees.
  • Fig. S2. Distribution of the expected frequency for different lineage tree founder compartments.
  • Fig. S3. Characterization of MS lesions and infiltration.
  • Fig. S4. Conventional sequencing shows that B cells present in the MS brain parenchyma and choroid plexus are clonally related to those present in the secondary lymphoid organs.
  • Fig. S5. Alignment of variable regions from representative B cell clones.
  • Fig. S6. Mutation frequency distribution among isotypes.
  • Fig. S7. Sequences from the CNS and CLN both show significant evidence of antigen-driven selection.
  • Fig. S8. IGHV family usage in the CNS and CLN.
  • Fig. S9. IGHV gene usage in the CNS and CLN.
  • Fig. S10. IGHJ gene usage in the CNS and CLN.
  • Fig. S11. Complete set of multicompartment lineage trees with unmutated single-tissue founders.
  • Fig. S12. Possible models of B cell expansion observed between the CLN and CNS.
  • Table S1. Nucleotide substitution matrix for clonal assignment.
  • Table S2. High-throughput sequencing results.
  • Table S3. Distribution of Ig isotypes.

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