Supplementary Materials

Supplementary Material for:

Immunoglobulin class-switched B cells form an active immune axis between CNS and periphery in multiple sclerosis

Arumugam Palanichamy, Leonard Apeltsin, Tracy C. Kuo, Marina Sirota, Shengzhi Wang, Steven J. Pitts, Purnima D. Sundar, Dilduz Telman, Lora Z. Zhao, Mia Derstine, Aya Abounasr, Stephen L. Hauser, H.-Christian von Büdingen*

*Corresponding author. E-mail:

Published 6 August 2014, Sci. Transl. Med. 6, 248ra106 (2014)
DOI: 10.1126/scitranslmed.3008930

This PDF file includes:

  • Fig. S1. Sagittal cervical spinal cord MRI of patient 14711.
  • Fig. S2. Bioinformatics pipeline for Ig-VH analyses.
  • Fig. S3. Numbers of Ig-VH in CSF and B cell subsets approximate expected levels of antigen stimulation.
  • Fig. S4. Bicompartmental clusters relative to total PB B cell clusters.
  • Fig. S5. Ig-VH amino acid sequence alignments of bicompartmental clusters shown in Fig. 2 (separate PDF file).
  • Fig. S6. IGHV usage profiles of CSF Ig-VH.
  • Fig. S7. Gating controls (single-color histograms) for CD19, CD20, CD27, CD38, and IgD flow cytometry.
  • Table S1. Sorted cells and sequence counts.
  • Table S2. PB B cell clusters connecting to the CSF/CNS compartment.
  • Table S3. Number of bicompartmental clusters by patient and B cell subset expressing Ig-VH related to CSF Ig-VH.
  • Table S4. Correlations between number of bicompartmental clusters found and CSF metrics.
  • Table S5. Bicompartmental clusters in proportion to total PB B cell clusters of the indicated PB B cell subset.
  • Table S7. Comparisons of numbers of reads in each nonredundant Ig-VH sequence group between naïve PB B cells and all other B cell subsets and CSF per patient.

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Other Supplementary Material for this manuscript includes the following:

  • Table S6. IGHV usage data used to generate heatmaps displayed in Fig. 3 (separate Excel file).
  • Table S8. SHM data used to generate heatmaps displayed in Fig. 4 (separate Excel file).

[Download Tables S6 and S8]