Supplementary Materials

Supplementary Material for:

An annexin A1–FPR1 interaction contributes to necroptosis of keratinocytes in severe cutaneous adverse drug reactions

Nao Saito, Hongjiang Qiao, Teruki Yanagi, Satoru Shinkuma, Keiko Nishimura, Asuka Suto, Yasuyuki Fujita, Shotaro Suzuki, Toshifumi Nomura, Hideki Nakamura, Koji Nagao, Chikashi Obuse, Hiroshi Shimizu,* Riichiro Abe*

*Corresponding author. E-mail: (R.A.); (H.S.)

Published 16 July 2014, Sci. Transl. Med. 6, 245ra95 (2014)
DOI: 10.1126/scitranslmed.3008227

This PDF file includes:

  • Fig. S1. Causative drug–specific lymphocytes in patients’ peripheral blood.
  • Fig. S2. The cytotoxicity of supernatant from DIHS/DRESS PBMCs.
  • Fig. S3. The cytotoxicity of supernatant from irrelevant drug–exposed SJS/TEN PBMCs.
  • Fig. S4. The cytotoxicity of keratinocytes from normal-appearing postlesional skin and nonlesional skin.
  • Fig. S5. Protein levels of necroptosis signaling molecules in keratinocytes from SJS/TEN patients, ODSR patients, or healthy controls.
  • Fig. S6. Effect of poly(I:C), TNF-α, and granulysin on SJS/TEN keratinocyte cytotoxicity.
  • Fig. S7. The cytotoxicity of CD14bright CD16 and CD14dim CD16+ cells on SJS/TEN keratinocytes.
  • Fig. S8. FPR1 stimulation does not induce phosphorylation of JNK, p38, or ERK.
  • Fig. S9. Cytotoxicity in SJS/TEN keratinocytes, ODSR keratinocytes, or healthy control keratinocytes induced by PBMC supernatant, as measured by LDH assay.
  • Table S1. Mass spectrometry result of the proteins in SJS/TEN supernatant.
  • Table S2. Patient and healthy control information.
  • Data File S1. The promoter region of FPR1 has no pathogenic mutations.
  • Reference (31)

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