Supplementary Materials

Supplementary Material for:

Biological pacemaker created by minimally invasive somatic reprogramming in pigs with complete heart block

Yu-Feng Hu, James Frederick Dawkins, Hee Cheol Cho, Eduardo Marbán,* Eugenio Cingolani*

*Corresponding author. E-mail: (E.C.); (E.M.).

Published 16 July 2014, Sci. Transl. Med. 6, 245ra94 (2014)
DOI: 10.1126/scitranslmed.3008681

This PDF file includes:

  • Fig. S1. HR trends, diurnal changes, and physical activity data from a TBX18-transduced animal without backup electronic pacing, superimposed on the corresponding pooled data for the routine protocol.
  • Fig. S2. TBX18-transduced cardiomyocytes for quantification.
  • Fig. S3. Cx43 expression in iSAN cells.
  • Fig. S4. Schematic of the experimental protocol in the porcine model of complete heart block.
  • Fig. S5. Injection site images.
  • Table S1. Baseline characteristics of GFP and TBX18 groups.
  • Table S2. Comparison of HRV between TBX18 and GFP animals.
  • Table S3. Staining conditions for HCN4.
  • Table S4. Primer sequences for the different genes studied in reprogrammed cells.

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