Supplementary Materials

Supplementary Material for:

Ultrahigh dose-rate FLASH irradiation increases the differential response between normal and tumor tissue in mice

Vincent Favaudon,* Laura Caplier, Virginie Monceau, Frédéric Pouzoulet, Mano Sayarath, Charles Fouillade, Marie-France Poupon, Isabel Brito, Philippe Hupé, Jean Bourhis, Janet Hall, Jean-Jacques Fontaine, Marie-Catherine Vozenin

*Corresponding author. E-mail: vincent.favaudon@curie.fr

Published 16 July 2014, Sci. Transl. Med. 6, 245ra93 (2014)
DOI: 10.1126/scitranslmed.3008973

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Setup for LINAC irradiation of mouse thorax.
  • Fig. S2. Time-dependent formation of the MV2+ radical at 603 nm.
  • Fig. S3. Optical detection of the formation of the MV2+ radical.
  • Fig.S4. Time course of the evolution of the methyl viologen MV2+ and thiocyanate (SCN)2 radicals.
  • Fig. S5. Correlation between methyl viologen and ferrous sulfate (Fricke) dosimeters.
  • Fig. S6. Gafchromic film imaging of the irradiation fields used in this study.
  • Fig. S7. Side view showing the positioning of animals for γ-ray irradiation of the chest.
  • Fig. S8. Depth-dose distribution of 4.5-MeV electrons and 137Cs γ-rays.
  • Fig. S9. Development of fibrosis foci after bilateral thorax irradiation of C57BL/6J mice.
  • Fig. S10. Immunohistochemical characterization of the TGF-β1/TGF-βRII/SMAD4 signaling cascade in the lungs of C57BL/6J mice at 24 weeks pi.
  • Fig. S11. Summary of the macroscopic lesions in C57BL/6J mice 24 weeks pi at the doses indicated.
  • Fig. S12. Morphology of HBCx-12A and HEp-2 tumor xenografts transplanted into Swiss nude mice.
  • Fig. S13. Illustration of the statistical analysis of tumor growth.
  • Table S1. Physical and biological properties of the photon and particle radiation generated by the facilities in this study.
  • Table S2. Mean partial pressure and concentration of oxygen in some normal tissues and tumors.
  • References (4353)

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