Supplementary Materials

Supplementary Material for:

Protein misfolding, congophilia, oligomerization, and defective amyloid processing in preeclampsia

Irina A. Buhimschi,* Unzila A. Nayeri, Guomao Zhao, Lydia L. Shook, Anna Pensalfini, Edmund F. Funai, Ira M. Bernstein, Charles G. Glabe, Catalin S. Buhimschi

*Corresponding author. E-mail: irina.buhimschi@nationwidechildrens.org

Published 16 July 2014, Sci. Transl. Med. 6, 245ra92 (2014)
DOI: 10.1126/scitranslmed.3008808

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Quantitative relationship of urine congophilia with 24-hour proteinuria.
  • Fig. S2. Western blot validation of protein identities associated with the PE misfoldome.
  • Fig. S3. Comparative aggregation prediction of interferon-inducible protein 6-16 (G1P3) isoforms.
  • Fig. S4. Effect of albumin depletion on ALZ90 immunoreactivity in preeclamptic urine.
  • Fig. S5. Fractional excretion and serum concentration of sAPP measured by ELISA.
  • Fig. S6. Patterns of APP immunoreactivity in preeclamptic urine.
  • Fig. S7. Immunolocalization of ALZ90 and G1P3 epitopes in calcified Alzheimer’s disease–like plaques in preeclamptic placenta.
  • Fig. S8. Patterns of immunoreactivity for β-secretases BACE1 and BACE2 in preeclamptic placenta.
  • Table S1. Demographic, clinical, and outcome characteristics of women whose urine samples were used in the feasibility phase (n = 80).
  • Table S2. Demographic, clinical, and outcome characteristics of pregnant women whose urine samples were used in the cross-sectional validation phase (n = 516).
  • Table S3. Comparative prognostic accuracy for prediction of MIDPE among subjects in the validation phase with known pregnancy outcomes (n = 563).
  • Table S4. Summary of LC-MS/MS protein identification results.
  • Table S5. Aggregation parameters of interferon-inducible protein 6-16 (IFI6 or G1P3).
  • References (6570)

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Other Supplementary Material for this manuscript includes the following:

  • Table S6. Exact P values (provided as an Excel file).

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