Supplementary Materials

Supplementary Material for:

Neutralizing antibodies to HIV-1 envelope protect more effectively in vivo than those to the CD4 receptor

Amarendra Pegu, Zhi-yong Yang, Jeffrey C. Boyington, Lan Wu, Sung-Youl Ko, Stephen D. Schmidt, Krisha McKee, Wing-Pui Kong, Wei Shi, Xuejun Chen, John-Paul Todd, Norman L. Letvin, Jinghe Huang, Martha C. Nason, James A. Hoxie, Peter D. Kwong, Mark Connors, Srinivas S. Rao, John R. Mascola,* Gary J. Nabel*

*Corresponding author. E-mail: jmascola@nih.gov (J.R.M.); gary.nabel@sanofi.com (G.J.N.)

Published 2 July 2014, Sci. Transl. Med. 6, 243ra88 (2014)
DOI: 10.1126/scitranslmed.3008992

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Generation of mAbs specific for primate CD4 in mice after prime-boost immunization.
  • Fig. S2. Neutralization of HIV-1 by 2D5.
  • Fig. S3. The 2D5 epitope on domain 1 of CD4.
  • Fig. S4. Binding of PG9 to GnTI− 293S cells.
  • Fig. S5. Gating strategy for determining percent CD4 occupancy by 2D5.
  • Table S1. Crystallographic data collection and refinement statistics.
  • Table S2. mAb2D5/CD4 interactions.
  • Table S3. Hydrogen bonds and salt bridges between mAb2D5 and CD4.
  • References (5760)

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