Supplementary Material for:

TREM2 mutations implicated in neurodegeneration impair cell surface transport and phagocytosis

Gernot Kleinberger, Yoshinori Yamanishi, Marc Suárez-Calvet, Eva Czirr, Ebba Lohmann, Elise Cuyvers, Hanne Struyfs, Nadine Pettkus, Andrea Wenninger-Weinzierl, Fargol Mazaheri, Sabina Tahirovic, Alberto Lleó, Daniel Alcolea, Juan Fortea, Michael Willem, Sven Lammich, José L. Molinuevo, Raquel S�nchez-Valle, Anna Antonell, Alfredo Ramirez, Michael T. Heneka, Kristel Sleegers, Julie van der Zee, Jean-Jacques Martin, Sebastiaan Engelborghs, Asli Demirtas-Tatlidede, Henrik Zetterberg, Christine Van Broeckhoven, Hakan Gurvit, Tony Wyss-Coray, John Hardy, Marco Colonna, Christian Haass*

*Corresponding author. E-mail: christian.haass@dzne.lmu.de

Published 2 July 2014, Sci. Transl. Med. 6, 243ra86 (2014)
DOI: 10.1126/scitranslmed.3009093

This PDF file includes:

• Materials and Methods
• Fig. S1. Absence of TREM2 fragments containing C-terminal FLAG tag.
• Fig. S2. Analysis of maturation of TREM2.
• Fig. S3. Characterization of BV2 cells stably overexpressing human TREM2.
• Fig. S4. Characterization of novel sTREM2 ELISA.
• Fig. S5. Impaired phagocytosis by mutant TREM2.
• Table S1. Primers used for RT-PCR analysis.
• Table S2. Characterization of novel sTREM2 ELISA.
• Table S3. Spike recovery and linearity test for CSF and plasma sTREM2 ELISA.
• References (44–48)

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