Supplementary Materials

Supplementary Material for:

Selective Targeting of TGF-β Activation to Treat Fibroinflammatory Airway Disease

Shunsuke Minagawa, Jianlong Lou, Robert I. Seed, Anthony Cormier, Shenping Wu, Yifan Cheng, Lynne Murray, Ping Tsui, Jane Connor, Ronald Herbst, Cedric Govaerts, Tyren Barker, Stephanie Cambier, Haruhiko Yanagisawa, Amanda Goodsell, Mitsuo Hashimoto, Oliver J. Brand, Ran Cheng, Royce Ma, Kate J. McKnelly, Weihua Wen, Arthur Hill, David Jablons, Paul Wolters, Hideya Kitamura, Jun Araya, Andrea J. Barczak, David J. Erle, Louis F. Reichardt, James D. Marks, Jody L. Baron, Stephen L. Nishimura*

*Corresponding author. E-mail: stephen.nishimura@ucsf.edu

Published 18 June 2014, Sci. Transl. Med. 6, 241ra79 (2014)
DOI: 10.1126/scitranslmed.3008074

This PDF file includes:

  • Materials and Methods
  • Fig. S1. ITGB8 BAC Tg mice express αvβ8 at similar expression levels and tissue distribution to humans.
  • Fig. S2. ITGB8 BAC transgene rescues early lethality of mouse itgb8 deficiency.
  • Fig. S3. Secreted human αvβ8 integrin–placental AP fusion proteins bind to murine latency-associated peptide.
  • Fig. S4. Dose response of B5 antibody treatment of intratracheal Ad-IL-1β–injected B-line BAC ITGB8 Tg mice.
  • Fig. S5. Antibody treatment with B5 does not have any effect on lung morphology.
  • Fig. S6. Cigarette smoke and poly(I:C) synergistically produce airway disease that resembles COPD in humans.
  • Fig. S7. Effects of combined exposure of cigarette smoke and poly(I:C) on inflammation and inflammatory mediators.
  • Fig. S8. B5 antibody treats allergic airway inflammation.
  • Fig. S9. Electron microscopy of integrin αvβ8.
  • Fig. S10. β8 antibody epitope mapping.
  • Fig. S11. Non–function-blocking antibodies binding to the Psi, hybrid, or epidermal growth factor (EGF) 1–2 domains.
  • Fig. S12. Genome-wide comparison of the effects of β8 and TGF-β neutralizing antibodies on human fibroblast gene expression.
  • Fig. S13. VH and VL sequences of 37E1 and B5.
  • Fig. S14. B5 improves the ability of 37E1 to inhibit the binding of soluble αvβ8 to latency-associated peptide.
  • Fig. S15. B5 specifically blocks binding of αvβ8, and not αvβ6, to latency-associated peptide.
  • Fig. S16. Gel filtration of clasped or unclasped αvβ8 in complex with B5 Fab.
  • Fig. S17. Electron microscopy of integrin αvβ8.
  • Table S1. Fibroblast differentially expressed gene array data.
  • Table S2. Autocrine TGF-β activation mediated by αvβ8 in human fetal tracheal fibroblasts.
  • References (5262)

[Download PDF]