Supplementary Materials

Supplementary Material for:

Chitinase 3–Like 1 Suppresses Injury and Promotes Fibroproliferative Responses in Mammalian Lung Fibrosis

Yang Zhou, Hong Peng, Huanxing Sun, Xueyan Peng, Chuyan Tang, Ye Gan, Xiaosong Chen, Aditi Mathur, Buqu Hu, Martin D. Slade, Ruth R. Montgomery, Albert C. Shaw, Robert J. Homer, Eric S. White, Chang-Min Lee, Meagan W. Moore, Mridu Gulati, Chun Geun Lee, Jack A. Elias,* Erica L. Herzog*

*Corresponding author. E-mail: erica.herzog@yale.edu (E.L.H.); jack_elias@brown.edu (J.A.E.)

Published 11 June 2014, Sci. Transl. Med. 6, 240ra76(2014)
DOI: 10.1126/scitranslmed.3007096

This PDF file includes:

  • Fig. S1. Colocalization of CHI3L1 and pro-SPC in the human lung.
  • Fig. S2. Colocalization of CHI3L1 and EMR-1 in the human lung.
  • Fig. S3. Immunofluorescence analysis of IPF lung cytospins demonstrates that Texas red–labeled CHI3L1 (red) colocalizes with (A) CCL18 (green) and (B) iNOS (green).
  • Fig. S4. Logistic regression modeling was used to calculate an unadjusted ROC curve as well as an ROC curve adjusted for age, gender, % FVC predicted at baseline, and corrected DLCO.
  • Fig. S5. Relationship of CD206 monocytes and CHI3L1 in the IPF circulation.
  • Fig. S6. Localization of CHI3L1 expression.
  • Fig. S7. CHI3L1 regulates collagen deposition in a temporally specific manner.
  • Fig. S8. CHI3L1 transgene is induced with doxycycline.
  • Fig. S9. Alveolar injury is increased in BRP-39−/− mice and is decreased in YKL-40 Tg mice.
  • Fig. S10. Mortality data.
  • Fig. S11. Wild-type and BRP-39 mutant mice were subjected to intratracheal bleomycin administration.
  • Fig. S12. Structural cell–derived CHI3L1 is required to prevent cell death, and bone marrow–derived CHI3L1 amplifies lung fibrosis.
  • Fig. S13. Wild-type and YKL-40 Tg mice underwent intratracheal bleomycin administration, and YKL-40 Tg was activated from days 5 to 14.
  • Fig. S14. H&E images of fibroblast-seeded mouse lung scaffolds grown in the presence of (left to right) CD206hi/lo macrophages, no macrophages, CD206hi macrophages, and CD206lo macrophages obtained from bleomycin-exposed mouse lungs.
  • Table S1. Characteristics of subjects for CHI3L1 analysis.
  • Table S2. Characteristics of subjects for CD206 analysis.
  • Table S3. Characteristics of progressive and stable IPF subjects for CD206 analysis.

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