Supplementary Materials

Supplementary Material for:

Hematopoietic Stem Cell Origin of BRAFV600E Mutations in Hairy Cell Leukemia

Stephen S. Chung, Eunhee Kim, Jae H. Park, Young Rock Chung, Piro Lito, Julie Teruya-Feldstein, Wenhuo Hu, Wendy Beguelin, Sebastien Monette, Cihangir Duy, Raajit Rampal, Leon Telis, Minal Patel, Min Kyung Kim, Kety Huberman, Nancy Bouvier, Michael F. Berger, Ari M. Melnick, Neal Rosen, Martin S. Tallman, Christopher Y. Park,* Omar Abdel-Wahab*

*Corresponding author. E-mail: (C.Y.P.); (O.A.-W.)

Published 28 May 2014, Sci. Transl. Med. 6, 238ra71 (2014)
DOI: 10.1126/scitranslmed.3008004

This PDF file includes:

  • Materials and Methods
  • Fig. S1. HSPC abnormalities in HCL and the presence of BRAFV600E in HSCs.
  • Fig. S2. Effect of BRafV600E expression at different time points and stages of hematopoiesis.
  • Fig. S3. Effect of BRafV600E expression on fetal hematopoiesis and in mice with B cell–restricted expression of the mutant allele.
  • Fig. S4. Effect of BRafV600E expression on HSPC numbers and frequencies.
  • Fig. S5. Effect of BRafV600E expression on B cell development and self-renewal.
  • Fig. S6. Normalization of HSPC compartment and increased myeloid/erythroid output from BRAFV600E-mutant HSPCs after BRAF inhibition.
  • Table S1. Genotyping results of cell populations sorted from HCL patient BM aspirates.
  • Table S2. Three hundred genes sequenced in three HCL leukemic cell and granulocyte genomic DNA samples.

[Download PDF]